反式HDV核酶对肝癌细胞端粒酶活性抑制的研究  

Inhibition on telomerase activity in heptocellular carcinoma by HDV ribozyme

作　　者：吕盈盈[1] 袁孟彪[1] 靳大川[1] 高艳景[1] 

机构地区：[1]北京协和医院消化内科,100730

出　　处：《临床内科杂志》2003年第9期487-489,共3页Journal of Clinical Internal Medicine

基　　金：山东省优秀青年科学基金资助

摘　　要：目的　观察HDV核酶是否能有效切割肝癌细胞端粒酶组分及在细胞内使端粒酶活性抑制。方法　设计合成针对端粒酶组分的HDV核酶的序列 ,构建该核酶的体外转录和真核表达质粒 ,测定HDV核酶对端粒酶的体外切割效力和在细胞内对端粒酶活性的抑制。结果　HDV核酶对底物的最大体外剪切效率为 70 %。导入细胞后 ,使端粒酶活性下降 90 %。结论　HDV核酶 (g .RZ5 7)对人肝癌细胞端粒酶活性有抑制作用 。Objective To observe the cleavage activity of HDV ribozyme for human telomerase RNA(hTR) component, and inhibition effect on telomerase activity.Methods Psedo-knotted g.RZ57 was designed and synthesized.An in vitro transcription plasmid and a eukaryotic expression plasmid of ribozyme were constructed.Then the cleavage activity of ribozyme against hTR RNA was determined in vitro and telomerase activity was detected in the cells.Results HDV ribozyme showed a specific cleavage activity against the telomerase RNA.The maximum cleavage ratio reached about 70%.The telomerase activity dropped by 90%.Conclusion HDV ribozyme(g.RZ57) can be potentially used as inhibitor for cancer therapy.

关 键 词：肝癌 治疗 端粒酶 反式HDV核酶 

分 类 号：R735.7[医药卫生—肿瘤]