乙醇对NIH3T3成纤维细胞成脂分化的影响  

An experimental study of ethanol-induced regulation of adipogenesis in NIH 3T3 fibroblasts

作　　者：李月白[1] 殷力[2] 王义生[2] 崔全军[3] 

机构地区：[1]郑州大学基础医学院生物化学教研室,郑州450052 [2]郑州大学第一附属医院骨科,郑州450052 [3]美国弗吉尼亚大学医院骨科Charlottesville,vausa22908

出　　处：《郑州大学学报（医学版）》2003年第5期758-760,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的 :观察乙醇对NIH 3T3成纤维细胞分化为脂肪细胞的作用 ,及其对成脂转录因子PPARγ表达的影响。方法 :以不同浓度乙醇作为诱导剂处理NIH 3T3成纤维细胞 14d ,苏丹Ⅳ染色 ,采用电子计算机图像分析软件ImageProPlus 4 .1,确定脂肪细胞百分比。采用逆转录聚合酶链式反应 (RT PCR)技术 ,检测细胞PPARγmRNA的表达。结果 :以递增浓度 (0 .0 3mol/L、0 .0 6mol/L、0 .0 9mol/L、0 .15mol/L、0 .2 1mol/L)乙醇处理NIH 3T3成纤维细胞 14d ,在 0 .0 6mol/L、0 .0 9mol/L、0 .15mol/L、0 .2 1mol/L乙醇组中 ,脂肪细胞百分比和PPARγmRNA表达均比对照组明显增高 ,差异均有统计学意义 (P <0 .0 0 1)。 0 .0 3mol/L乙醇组与对照组间的脂肪细胞百分比和PPARγmRNA表达差异均无统计学意义 (P >0 .0 5 )。结论 :乙醇能够直接诱导NIH 3T3成纤维细胞大量分化为脂肪细胞 。Aim:To observe the effects of ethanol on the differentiation of NIH 3T3 fibroblasts into adipocytes, and the changes of expression of an adipogenic transcription factor, PPARg, in the cells treated with ethanol.Methods:NIH 3T3 fibroblasts were cultured in vitro and treated with ethanol that acted as adipocyte differentiation inducer. The cells in culture were stained with Sudan IV. The percentage of adipocytes was calculated by using computer image analysis software, Image Pro Plus 4.1. The expression of PPARγ mRNA was investigated by means of reverse transcription polymerase chain reaction (RT PCR). Results:The cells in culture were treated with increasing (0.03 mol/L, 0.06 mol/L, 0.09 mol/L, 0.15 mol/L, 0.21 mol/L) concentrations of ethanol for 14 days. Both percentages of adipocytes and the expressions of PPARγ mRNA in the cells treated with 0.06 mol/L, 0.09 mol/L, 0.15 mol/L, and 0.21 mol/L ethanol markedly increased compared with control group ( P <0.001). The differences in the percentage of adipocytes and the expression of PPARγ mRNA between 0.03 mol/L ethanol group and control group were not statistically significant ( P >0.05). Conclusion:Ethanol can directly induce the differentiation of NIH 3T3 fibroblasts into a large number of adipocytes, which might be one of the factors contributing to the increase in volume of fat in the marrow following the ethanol induced osteonecrosis.

关 键 词：乙醇 NIH3T3成纤维细胞 细胞分化 脂肪细胞 乙醇性骨坏死 

分 类 号：R364.3[医药卫生—病理学] R681[医药卫生—基础医学]