白藜芦醇对大鼠腔隙性脑梗死模型急性期的脑保护作用  

The neuroprotective effect of resveratrol during acute period of lacunar infarction model in rats

作　　者：刘茅茅[1] 王拥军[1] 李小玲[2] 梁宪红[1] 梁德军[1] 

机构地区：[1]首都医科大学附属北京天坛医院神经内科,北京100050 [2]美国约翰霍普金斯大学医学院麻醉科

出　　处：《郑州大学学报（医学版）》2003年第5期731-733,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：人事部留学回国人员科技活动择优资助项目 2 0 0 1 119

摘　　要：目的 :探讨白藜芦醇 (RES)对大鼠腔隙性脑梗死 (LI)模型急性期的脑保护作用。方法 :6 0只雄性SD大鼠 ,随机分为 4组 ,按照Toshima腔隙性脑梗死模型的制作方法 ,于实验第 1d、3d右颈内动脉内注射 10 0 μg月桂酸钠建立LI模型 ,其中 3组每d 1次尾静脉内注射不同剂量的RES(剂量分别为 1μg/kg、0 .1μg/kg、0 .0 1μg/kg) ,第 4组只注射等量溶剂作为对照。采用姿势反射试验评价神经功能缺损 ,组织病理学分析脑梗死面积。结果 :第 2次注入月桂酸钠后 72h(实验第 6d) ,1μg/kg ,0 .1μg/kg ,0 .0 1μg/kgRES剂量组及对照组中 ,有神经功能缺损的大鼠分别占 4 6 .7%、4 0 .0 %、73.3%、73.3% ,4组数据行 χ2 检验 ,总体差异无统计学意义 (P >0 .0 5 ) ,1μg/kg ,0 .1μg/kgRES组有减少LI模型脑梗死面积的趋势 ,但总体差异无统计学意义 (P >0 .0 5 )。结论 :RES在LI急性期有减少LI面积 ,改善神经功能缺损的可能 。Aim:To explore whether resveratrol (RES) could effectively suppress infarct size or improve neurological deficits during acute period in the Toshima's lacunar infarction (LI) model.Methods:A total of 60 male SD rats were randomly divided into 4 groups. According the methods of Toshima's LI model, 100 μg of sodium laurate was injected into the right internal carotid artery at day 1 and 3 respectively to develop LI model. RES was administered to 3 groups at different doses (the doses of RES were 1 μg/kg,0.1 μg/kg,0.01 μg/kg) via intravenous injection once a day from day1 to day 5, The 4th group was administered the same dose vehicle only. The consequent of 4 groups were examined by posture reflex test and histopathological analyses. Results:72 hours after the second injection of sodium laurate (day 6), 46.7%,40%,73.3%,73.3% rats showed mild to severe neurological deficits in 1 μg/kg,0.1 μg/kg,0.01 μg/kg and control groups respectively. We could find a trend which is RES could reduce the area of infarction under the concentration of 1 μg/kg,0.1 μg/kg, but there was no significant difference between these 4 groups ( P >0.05). Conclusion:It is possible that RES could reduce the infarction area and improve neurological deficits in the acute period of LI. Further study about mechanism of RES needs to be done in the future.

关 键 词：脑梗死 白藜芦醇 大鼠 急性期 脑保护 

分 类 号：R743.33[医药卫生—神经病学与精神病学]