脆性组氨酸三联体基因异常转录与肝癌关系的研究  

Relationship between aberrant FHIT transcripts and hepatocellular carcinoma

作　　者：于永春[1] 孙奋勇[2] 李智[1] 卢少毅[1] 滕小洪[1] 

机构地区：[1]同济大学附属铁路医院中心实验室,上海200072 [2]暨南大学生物工程研究所

出　　处：《中华肝脏病杂志》2003年第9期550-551,共2页Chinese Journal of Hepatology

基　　金：铁道部医学专项基金(TW200027)

摘　　要：目的 研究脆性组氨酸二联体(FHIT)基因异常转录与肝细胞癌(HCC)的相关性。方法利用逆转录-聚合酶链反应(RT-PCR)和单链构象多态性(SSCP)技术检测24对肝癌及癌旁组织、4例正常肝组织中FHIT的转录和突变情况。结果 11例肝癌组织(46％)标本、2例(8％)癌旁正常组织检测到异常转录本,正常肝组织无异常转录。结论 FHIT基因异常转录可能是导致肝细胞癌形成的一个重要因素。Objective To study the relationship between aberrant FHIT transcripts and hepatocellular carcinoma (HCC), Methods Reverse transcription-polymerase chain reaction (RT-PCR) and single strand conforma-tional polymorphism (SSCP) assays were used to analyze the transcripts and mutations of FHIT gene in 24 matched tumorous tissues and para-tumorous tissues from patients with HCC and in 4 normal liver tissues. Results Aberrant FHIT transcripts were observed in 11 out of 24 (46%) tumorous tissues and in 2 (8%) of the matched para-tumorous tissues. Conclusion FHIT aberrant transcripts may play an important role in the pathogenesis of hepatocellular carcinoma.

关 键 词：脆性组氨酸三联体 基因异常转录 肝癌 基因表达 聚合酶链反应 单链构象多态性 

分 类 号：R735.7[医药卫生—肿瘤]