青枯病原细菌无毒菌株诱导番茄防御酶系及粗提液抑菌活性的影响  

The effects of induced resistance to bacterial wilt by avirulent strains on the defense enzymes and inhibitory activities of crude extracts in tomato leaves

作　　者：陈庆河[1] 翁启勇[1] 胡方平[2] 

机构地区：[1]福建省农科院植保所,福州350013 [2]福建农林大学植保学院,福州350003

出　　处：《植物病理学报》2003年第4期334-337,共4页Acta Phytopathologica Sinica

基　　金：福建省自然科学基金资助项目 (B0 2 1 0 0 31 ) ;福建省科技厅重大项目 (2 0 0 0Z 0 0 6)

摘　　要：用紫外诱变法获得的青枯无毒菌株诱导番茄 ,研究了诱导番茄细胞内防御酶系及组织粗提液抑菌活性的影响。结果表明 :番茄经无毒菌株处理后 ,体内与抗病反应相关的苯丙氨酸解氨酶 (PAL)、过氧化物酶 (POD)、多酚氧化酶 (PPO)及超氧化物歧化酶 (SOD)等防御酶系活性均显著增加。无毒菌株可诱导产生植株体外和体内对致病菌有抑制作用的抑菌物质。表明青枯无毒菌株产生诱导抗性的机制可能是激活了植物本身的抗病代谢过程。Avirulent strains of Ralstonia solanacearum (Atm044 and Asp061) obtained by UV mutagenesis were studied on resistance induction on tomato plants. After the inoculation of the avirulent strains, a significant increasing of the activities of peroxidase (POD),phenylanine ammonia lyase (PAL),polyphenoloxidase (PPO) and superoxide dismutase (SOD) in tomato plants was observed. The crude extracts from avirulent strain treated plants showed a strong inhibitory effect on virulent strains in vivo as well as in vitro . Our results indicated that the avirulent R.solanacearum strains induced tomato resistance possibly through triggering the plant defense reactions.

关 键 词：青枯病 病原细菌 无毒菌株 基因诱导 番茄 防御酶系 粗提液 抑菌活性 影响因素 紫外诱变法 

分 类 号：S436.412.15[农业科学—农业昆虫与害虫防治]