农杆菌介导GNA基因转化菜薹  

Genetic Transformation of Flowering Stalk with Snowdrop Lectin Gene(GNA)

作　　者：张扬勇[1] 李汉霞[1] 叶志彪[1] 卢永恩[1] 陆芽春[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室

出　　处：《园艺学报》2003年第4期473-475,共3页Acta Horticulturae Sinica

摘　　要：以菜薹带柄子叶为外植体 ,采用农杆菌介导法将特异启动子PRSs-1与雪花莲凝集素 (GNA)基因构建的嵌合基因转化菜薹 ,获得 2 6株Km抗性植株 ,经过PCR检测和PCRCotyledons with petiole of Flowering stalk were transformed with the vector carrying a snowdrop lectin gene( Galanthus nivalid Agglutinin, GNA ).Twenty six transformants were obtained from cotyledons of Flowering Stalk.They were confirmed to be transformants by the PCR and PCR Southern blot analysis.

关 键 词：农杆菌介导 GNA基因 基因转化 菜薹 带柄子叶 外植体 雪花莲凝集素 基因构建 嵌合基因 

分 类 号：S634.5[农业科学—蔬菜学]