抗重组EB病毒抗原双重抗体检测血清学诊断鼻咽癌的研究(英文)  

Study on Sero-Diagnosis of Nasophar yngeal Carcinoma Using a Dual Antibody Test against Recombinant E pstein-Barr Virus Antigens

作　　者：顾耀亮 张昌卿[2] 吴子柏[3] 宗永生[4] 梁英杰[4] 陈跃龙 

机构地区：[1]中山生物工程有限公司,广东中山528437 [2]中山大学肿瘤防治中心实验研究部,广东广州510060 [3]香港大学医学院微生物系 [4]中山大学中山医学院病理学教研室,广东广州510089

出　　处：《癌症》2003年第9期903-906,共4页Chinese Journal of Cancer

摘　　要：背景与目的:在评估4种EB病毒抗原酶联免疫吸附法的基础上,探讨优化抗重组EB病毒抗原双重抗体检测应用于血清学诊断鼻咽癌。方法:收集广州地区57例治疗前鼻咽癌患者和58例健康成人的血清。应用EB病毒特异抗原(谷胱甘肽转移酶重组融合蛋白)为基础的4种免疫酶联吸附法,即EBNA1-IgA,EBNA1-IgG,Zta-IgA和Zta-IgG检测血清中抗EB病毒的抗体水平。结果:EBNA1-IgA的灵敏度(0.9123)和阴性预测值(0.9074)是单独使用4种ELISA实验中最高的。Zta-IgA具有最高的正确率(л,0.8870)和Youden指数(J,0.7738)。当评估配对的ELISA时,EBNA1-IgA和Zta-IgA双重阳性的所有指标是4种双重阳性实验中最高的。5例EBNA1-IgA阴性的鼻咽癌患者呈Zta-IgA阳性,而7例Zta-IgA阴性的鼻咽癌患者呈EBNA1-IgA阳性。结论:EBNA1-IgA酶联免疫吸附的单独检测在血清学诊断鼻咽癌时优于其他3项(EBNA1-IgG、Zta-IgA和Zta-IgG)单独酶联免疫吸附检测。EBNA1-IgA和Zta-IgA两项的组合应用在血清学诊断鼻咽癌时有互补作用,是血清学检测的合适组合。BACKGROUND &OBJECTIVE:The aim of this study was to optimize a dual-antibody assay for s ero-diag nosis of nasopharyng eal ca rcinoma (NPC)by evaluating 4Epstein-Barr virus (EBV)antig en-based enzyme-linked immunosorbent assays(ELISAs ).METHODS :The serum samples of 57pretreated NPC patients and 58appar ently healthy adults in Guang zhou we re collected.The levels of anti-EBV antibody in the sera were tested by 4ELI SAs ,which were developed using fusion pr oteins of g lutathione transferase a nd EBV specific recombinant antig ens ,nam ely,EBNA1-Ig A,EBNA1-Ig G,Zta-Ig A,and Zta-Ig G.RESULTS :When evaluated individually,the se nsitivity(0.9123)and neg ative predictive value(0.9074)of EBNA1-Ig A were the hig hest among t he 4ELISAs tested.Zta-Ig A test had the h ig hest individual accuracy rate(л,0.8870)and Youden index(J ,0.7738).The dual positives for EBNA1-Ig A an d Zta-Ig A were the hig hest among the 4dual posi tives when paired ELISAs were evaluated.Five NPC patients with neg ative reaction to EBNA1-Ig A showed positive re action of Zta-Ig A,and 7NPC patients with neg ative reaction to Zta-Ig A showed po sitive reaction of EBNA1-Ig A.CONCLUSION:The EBNA1-Ig A assay is more suitable than the other 3ELISAs (EBNA1-Ig G,Zta-Ig A,and Zta-Ig G)when used individually for serolog ical diag nosis of NPC.When two assays are combi ned,EBNA1-Ig A and Zta-Ig A have compleme ntary effect on serolog ical diag nos is for NPC and is thus an optimal combinatio n of serum antibody assays.

关 键 词：抗重组EB病毒抗原 双重抗体 检测 血清学诊断 鼻咽癌 

分 类 号：R739.63[医药卫生—肿瘤]