消减抑制杂交及其在肿瘤研究中的应用  

Suppression Subtractive Hybridiza tion and Its Application in Study of Tumors

作　　者：梁莉[1] 丁彦青[1] 石益民[1] 

机构地区：[1]第一军医大学病理教研室,广东广州510515

出　　处：《癌症》2003年第9期997-1000,共4页Chinese Journal of Cancer

基　　金：国家重点基础研究发展规划973项目(2001C13510208);国家自然科学基金(30170423);军队医药卫生基金(01MA128)

摘　　要：消减抑制杂交(suppressionsubtractivehybridization,SSH)是最近提出的一种以抑制PCR为基础的差异显示基因克隆技术。该方法具有简便易行、特异性高、能分离出丰度较低的特异性片段等优点,并已成功应用于肿瘤、发育生物学、免疫调控等方面的研究,本文简述其原理、方法、及在肿瘤研究中的应用进展,并对其方法的优缺点和应用前景进行分析。Suppression subtractive hybridiza tion(SSH)has been recently reported as a differential display t echnique at the basis of suppressive polymerase chain reaction(PCR).This technique has such advantag es as easy and feasible,hig hly specific,capa ble of separating the low abundant sp ecific frag ments.Now it has been successfu lly applied in the study of tumors ,developing biolog y,immune reg ulat ions ,etc.Here we summarized its principle,method,and advances in the study of t umors ,then analyzed its merits ,def ects,and applicable potentials.

关 键 词：消减抑制杂交 肿瘤 基因克隆 特异性片段 CDNA 

分 类 号：R730.2[医药卫生—肿瘤]