AFP异质体检测及其临床应用价值  

Detection of lectin-reactive alpha-fetoprotein and its clinical value

作　　者：李招云[1] 吴晓宇[1] 赖卫强[1] 莫经刚[1] 戴岳楚[1] 高琳[1] 

机构地区：[1]浙江省台州市中心医院,台州318000

出　　处：《中国免疫学杂志》2003年第9期651-653,共3页Chinese Journal of Immunology

基　　金：浙江省台州市科委科技项目 (No .0 0 2 2 0 9)资助

摘　　要：目的 :探讨甲胎蛋白 (AFP)异质体检测对原发性肝癌、良性肝病的临床应用价值。方法 :根据小扁豆凝集素对AFP亲和力不同 ,采用亲和免疫电泳印迹法检测AFP L3;化学发光免疫分析法检测AFP。结果 :以AFP L3≥ 15 %作为PHC的诊断标准 ,其敏感性为 91 1% ,与慢性肝病鉴别诊断的特异性为 95 .0 % ,AFP L3含量与血清AFP浓度及原发性肝癌癌灶大小无关。结论 :AFP L3结合AFP检测对原发性肝癌 ,尤其是小肝癌的早期诊断和与良性肝病的鉴别诊断具有重要的临床应用价值。Objective:To study the clinical value of lectin reactive alpha fetoprotein(AFP) detection in primary hepatocellular carcinoma (PHC) and benign liver diseases.Methods:According to the different affinity with AFP, AFP L 3 was detected by affinity immunoelectrophoresis blotting method, AFP detected by Chemiluminescent Immunoassay.Results:Taking AFP L 3≥15% as the diagnosis criteria of PHC,the sensitivity was 91.1%,the specificity in differential diagnosis in chronical liver diseases was 95.0%,the levels of AFP L 3 has no correlation to serum AFP levels and the sizes of PHC.Conclusion:It is of great clinical value to detect AFP L 3 and serum AFP levels for PHC in early diagnosis and benign differential diagnosis in liver diseases. [

关 键 词：AFP异质体 检测 临床应用 原发性肝癌 小扁豆凝集素 

分 类 号：R735.7[医药卫生—肿瘤]