β-呋喃果糖苷酶产生菌的筛选及产酶条件初探  

Selection of condition and bacteria species for producing β-Fructofuranosidase

作　　者：于国萍[1] 徐红华[1] 张英华[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《东北农业大学学报》2003年第3期322-325,共4页Journal of Northeast Agricultural University

基　　金：黑龙江省自然科学基金资助项目

摘　　要：β-呋喃果糖苷酶是一种具有转移活力的水解酶 ,该酶能以蔗糖和乳糖为底物 ,合成乳果糖。这是一种功能性低聚糖 ,对人体健康有益。实验研究了不同菌株产β-呋喃果糖苷酶活力的能力 ,确定节杆菌 A为最佳产酶菌株 ,对酶的生成具有诱导作用的碳源是蔗糖 ,培养时间为 4 0 h。Fructorfuranosidase was a hydrolase with transfer activity.This enzyme can use sucrose and Lactose as raw materials and then synthesize lactosucrose.Lactosucrose is a functional oligosacharide and is health for human body.In this paper,a fungus species was found that can produce β-Fructorfuranosidase,and Arthrobactor sp. was the best one.The best carbon souse-4% sucrose was selected,culturing time was 40 hour.

关 键 词：Β-呋喃果糖苷酶 菌株筛选 产酶条件 乳果糖 节杆菌 水解酶 功能性低聚糖 

分 类 号：Q556.2[生物学—生物化学] Q53