应用微卫星标记分析东乡野生稻遗传多样性初探  

Genetic Diversity of Dongxiang Wild Rice Detected by SSLP Markers

作　　者：谢建坤[1] 陈大洲[1] 肖叶青[1] 罗世友[1] 郑康乐[2] 庄杰云[2] 

机构地区：[1]江西省农业科学院水稻研究所,南昌330200 [2]中国水稻研究所国家水稻改良中心,杭州310006

出　　处：《中国农业科学》2003年第8期873-878,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金地方重点项目 (3 99690 0 1) ;国家"863"计划项目 (2 0 0 1AA2 2 715 2 );江西省自然科学基金资助项目 (0 3 3 0 0 3 4)

摘　　要：利用SSLP标记对东乡野生稻进行多样性分析。结果表明 ,在 14对SSLP标记引物中 ,12对引物扩增的产物呈现多态 ,多态性探针百分率为 85 .71% ,共扩增出 70条多态性带 ,平均每对引物扩增出多态性带 5 .83条 ,表明东乡野生稻具有较丰富的遗传多样性。聚类分析结果表明 ,东乡野生稻 9个居群内及居群间存在一些差异较大的材料 ,同时也存在一些相同或遗传距离很小的材料 ;居群内平均遗传距离为 0 .2 3～ 0 .4 7,居群间平均遗传距离为0 .4 0～ 0 .5 5 ,居群间的遗传距离略大于居群内的遗传距离 ,为此 ,必须对东乡野生稻现有居群进行严格的保护。Genetic diversity of Dongxiang wild rice was evaluated by using 14 SSR primer pairs. Twelve of the 14 SSR primer pairs amplified polymorphic bands. A total of 70 polymorphic DNA bands were generated with average polymorphic bands per primer pair of 5.83, indicating that the genetic diversity of Dongxiang wild rice was high. Clustering analysis showed that there were highly variant individuals within populations and among populations, while some others were highly similar with each other. Genetic distances of intrinsic-populations were 0.23-0.47, being smaller than the values of inter-populations which were 0.40-0.55. Strict measurements must be taken for the preservation of Dongxiang wild rice.

关 键 词：应用 微卫星标记分析 东乡野生稻 遗传多样性 SSLP标记引物 遗传距离 

分 类 号：S511.9[农业科学—作物学]