D-苯丙氨酸产生菌的诱变育种  

Improvement of D-phenylalanine producing strains by mutation

作　　者：李环[1] 韦萍[1] 顾海红[1] 欧阳平凯[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《工业微生物》2003年第3期6-10,共5页Industrial Microbiology

基　　金：国家十五攻关项目 2 0 0 1BA70 8B0 2 -0 6

摘　　要：先后使用紫外与 5 FU复合处理及Nd∶YAG倍频脉冲激光辐照等方法对D 苯丙氨酸产生菌PseudomonasputidaJS 0 1进行诱变 ,筛选到一株稳定高产的D 苯丙氨酸产生菌 10 0 3。对底物 5 苄基海因的转化率由 5 5 .3%上升到 85 .5 % ,提高率为 5 4.6 %。After the D Phenylalanine producing strain Pseudomonas putida JS 01 was mutagenized by ultraviolet ray+5 fluorouracil and Nd:YAG laser, a high yielding mutant 1003 was obtained. 85.5% substrate could be transformed to the product, which was 54.6% higher than that of the original strain. The transformation could keep steady at a higher substrate concentration.

关 键 词：D—苯丙氨酸 产生菌 诱变 育种 紫外线 激光 

分 类 号：TQ922[轻工技术与工程—发酵工程]