胰腺导管癌中血管生成、细胞增殖指数、DNA倍性检测的临床意义  

Clinical Implication of Detecting Angiogenesis,Cell Proliferating Index and DNA Ploidy in Tissue of Pancreatic Duct Adenocarcinoma

作　　者：陈建武 史桂英[2] 肖家诚[1] 

机构地区：[1]上海第二医科大学附属瑞金医院病理科 [2]上海第二医科大学生物物理教研组,200025

出　　处：《诊断学理论与实践》2003年第3期208-211,共4页Journal of Diagnostics Concepts & Practice

摘　　要：目的:探讨血管生成、细胞增殖指数、DNA倍性在胰腺导管癌发生、发展中的作用及临床意义。方法:对29例胰腺导管癌和7例慢性胰腺炎患者的石蜡包埋标本作苏木精鄄伊红(HE)染色,CD34、Ki67免疫组织化学染色,镜下计算微血管密度(MVD)及Ki67阳性细胞比例,同时用流式细胞仪(FCM)检测细胞核DNA倍性,计算异倍体出现率及S期比例,并结合临床病理资料对各项指标作相关分析。结果:胰腺导管癌和慢性胰腺炎都有较多的新生血管。MVD值在胰腺导管癌高、中、低分化3组之间差异无显著性。按肿瘤直径分为≤1.5cm、1.6～2.9cm、≥3cm3组,此3组间MVD值、Ki67阳性细胞百分率、DNA异倍体出现率及肿瘤周围有无浸润的差异都非常显著(P<0.01)。肿瘤越大,MVD值、Ki67阳性细胞百分率及DNA异倍体出现率越高。结论:胰腺导管癌的发生、发展伴血管生成和DNA合成增加,联合测定MVD、Ki67阳性细胞百分率、DNA倍型可能为胰腺导管癌临床预后预测提供辅助参考指标。To study the role of detecting angiogenesis,cell proliferating index(CPI),DNA ploidy in tissue of pancreatic duct adenocarcinoma(PDA).Methods:Surgical specimens of29cases of PDA and7case of chronic pancreatitis(CP)were investigated histologically:immunohistochemical staining for CD34,Ki67and flow cytometer(FCM)for DNA ploidy.Microvascular density(MVD),ratio of Ki67positive cells,ratio of aneuploidy and of S phase were determined under microscopy.Results:Many new capillaries were found in both DAP and CP.No significant difference was noted among the3groups of DAP(well,middle and poor differenciation),but there were significant differences in MVD,ratio of Ki67positive cells,ratio of aneuploidy and with or without infiltration of the tumors among the3groups according to the tumor sizes(≤1.5cm,1.6-2.9cm,≥3cm).The bigger the tumor,the higher the MVD,ratio of Ki67positive cells and ratio of aneuploidy(P<0.01).Conclusions:Angiogenesis and increased DNA synthesis may be associated the oncogenesis of DAP.The combination of determing MVD,CPI and DNA ploidy may be helpful for clinical prognosis of DAP.

关 键 词：胰腺导管癌 血管生成 细胞增殖指数 DNA倍性检测 

分 类 号：R735.9[医药卫生—肿瘤]