人参皂苷预处理对电离辐射下人造血干细胞的保护作用及其机制探讨  

Protective effect of ginsenoside on hematopoietic progenitor cells under ionizing radiation

作　　者：黄颖[1] 梁晓燕[1] 李骋进 胡军[1] 康晓莉[1] 

机构地区：[1]陕西省人民医院,西安710068

出　　处：《山东医药》2015年第8期1-3,共3页Shandong Medical Journal

基　　金：陕西省自然科学基金资助项目(S2014JC11060)

摘　　要：目的观察人参总皂苷预处理对电离辐射下人造血干细胞(HPCs)的保护作用,并探讨其作用机制。方法将HPCs随机分为对照组和观察组,对照组不处理,观察组予以人参皂苷5.0μmol/L培养24 h,分别用剂量为0、1、2、5 Gy的X线对两组细胞进行照射。MTT法测算细胞存活率,流式细胞仪测定辐射后24 h细胞内的活性氧(ROS),Western blot法测定细胞内的Caspase-3和Nrf-2。结果随着辐射剂量的增加,两组细胞存活率均逐渐下降(P均<0.05),细胞内ROS活性增强、Caspase-3表达增加、Nrf-2表达减少(P均<0.05);与相同照射剂量对照组比较,观察组细胞存活率增加(P均<0.05),细胞内ROS活性减弱、Caspase-3表达减少、Nrf-2表达增加(P均<0.05)。结论人参皂苷对电离辐射下的HPCs具有保护作用,该作用与其抗凋亡和抑制氧化应激有关。Objective To investigate the protective effect of ginsenoside on hematopoietic progenitor cells( HPCs)under different intensity of ionizing radiations. Methods The HPCs were divided into the control group and the observe group,then to add ginsenoside into the observe group and cultivated for 24 hours. Respectively exposed under X-ray irradiations on 1,2,5 Gy. Cell viability was studied by MTT method. The generation of reactive oxygen species( ROS) after 24 hours,the expression of caspase-3 mRNA and Nrf-2 mRNA were measured by means of flow cytometry and Western blot,respectively. Results With the increase of radiation dose,cell viability reduced,the generation of ROS increased,the expression of caspase-3 mRNA enhanced and the Nrf-2 mRNA decreased,gradually,all P < 0. 05. Compared with control group with the same radiation dose,cell viability in the observe group increased,the generation of ROS and the expression of caspase-3 mRNA decreased,the Nrf-2 mRNA increased,all P < 0. 05. Conclusion Ginsenoside could protect HPCs from radiation effects,which was correlated with anti-apoptosis and inhibition of oxidation stress.

关 键 词：人参皂苷 造血干细胞 电离辐射 活性氧 半胱氨酸蛋白酶 Nrf-2 

分 类 号：R730.55[医药卫生—肿瘤]