湖北汉族群体9个STR位点遗传多态性分析  

Analysis of Genetic Polymorphisms of Nine STR Loci in Hubei Han Population

作　　者：李霞[1] 周新[1] 李杰[1] 贺小玲[1] 刘芳[1] 郑芳[1] 

机构地区：[1]武汉大学中南医院基因诊断中心,武汉,430071

出　　处：《武汉大学学报（医学版）》2003年第4期307-311,共5页Medical Journal of Wuhan University

基　　金：湖北省重点科技攻关项目 (项目编号 :2 0 0 1AA3 0 5B0 6)

摘　　要：目的 :应用荧光标记短串联重复序列 (shorttandemrepeat,STR)位点复合扩增方法 ,了解湖北汉族人 9个STR位点 (D3S135 8、vWA、FGA、D8S1179、D2 1S11、D18S5 1、D5S818、D13S317、D7S82 0 )的遗传多态性分布。方法 :选取2 2 0名无血缘关系的湖北汉族个体。采用Chelex法提取毛球和血液DNA ,将各STR位点引物用三色荧光标记后进行复合扩增 ,PCR产物经PE310遗传分析仪自动分析 ,并进一步进行遗传多态性研究。结果 :9个STR位点基因型观察数 19～ 5 0个 ,等位片段数 7～ 16个 ,多态信息含量介于 0 .7335～ 0 .84 5 2之间 ,个人识别率介于 0 .8971～0 .95 6 4之间。 9个STR位点在中国汉族人群中的等位基因频率分布与白色人种、黑色人种之间差异有显著性。结论 :采用荧光标记引物进行复合扩增 ,结合PE310遗传分析仪自动分析 ,可以有效地区分各位点的扩增产物。 9个STR位点的基因型分布均符合Hardy Weinberg平衡 。Objective: To apply the method of multiplex PCR by using fluorescently labeled STR primers to investigate the genetic polymorphism at nine STR loci in Hubei Han population. Methods: 220 unrelated individuals of Hubei Han was involved. Their DNA samples were extracted with Chelex method and were co amplified by using fluorescently labeled STR primers. The PCR products were detected using PE310 genetic analyzer,and the distribution of allele frequencies of these nine STR loci in Han population was investigated. Results: The number of genotypes,alleles,polymorphism information content and discrimination power at these nine STR loci were 19～50,7～16,0.733 5～0.845 2 and 0.897 1～0.956 4 respectively. Statistical differences were observed concerning these nine loci compared with the US Caucasian population data and the African American data. Conclusion: By co amplification using fluorescently labeled STR primers and detecting using PE310 genetic analyzer,the PCR products of these nine loci can be significantly distinguished. The allele distribution of these nine STR loci is in good agreement with the Hardy Weinberg equilibrium. The obtained data are beneficial to understand the population genetics of the nine STR loci in Chinese Han population.

关 键 词：湖北 汉族群体 STR位点 遗传多态性 

分 类 号：Q75[生物学—分子生物学]