新型希夫碱钌配合物的抗肿瘤作用研究  

Study on antitumor effect of a novel schiff base ruthenium coordination compound

作　　者：赵成亮[1] 张宝琦[1] 杜元良[1] 闫鹤[2] 张四喜[2] 陈宾[1] 

机构地区：[1]承德医学院附属医院,河北承德067000 [2]吉林大学第一医院,吉林长春130021

出　　处：《中国生化药物杂志》2015年第8期31-33,37,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：河北省卫生厅科研基金(ZL20140116);吴阶平医学基金会临床科研专项资助基金(320.6750.14119)

摘　　要：目的探讨新型希夫碱钌配合物对胃癌SGC-7901细胞生长增殖和细胞凋亡的影响。方法将胃癌SGC-7901细胞根据不同浓度的新型希夫碱钌配合物处理分为10、30、50μmol/L组,另设DMSO空白组。MTT法检测新型希夫碱钌配合物对胃癌细胞增殖的影响,流式细胞术检测各组细胞凋亡及细胞周期变化。结果 MTT结果显示SGC-7901细胞生长抑制率随新型希夫碱钌配合物浓度的增加而增加,各剂量组在24、48、72 h的抑制率均显著高于空白组(P<0.05)。流式细胞术结果显示浓度为10、30、50μmol/L的新型希夫碱钌配合物组的细胞凋亡率分别为(17.64±1.21)%、(26.47±0.61)%、(55.63±1.49)%,均显著高于空白组(P<0.05)。随着新型希夫碱钌配合物浓度升高,G1期细胞所占的百分比逐渐增加。结论新型希夫碱钌配合物能够抑制胃癌SGC-7901细胞的增殖,促进细胞凋亡,并使细胞停滞在G1期。Objective To study effect of a novel schiff base ruthenium coordination compound on cell proliferation and apoptosis of gastric cancer SGC-7901 cell. Method Gastric cancer SGC-7901 cell were divided into four groups according to different treatment of novel schiff base ruthenium coordination compound( concentration of 10,30,50 μmol / L) and blank group with DMSO. Cell proliferation was detected by MTT assay,cell apoptosis and cell cycle were analysed by flow cytometry. Results MTT results showed the inhibitory effect of novel schiff base ruthenium coordination compound on SGC-7901 cell enhanced with the increase of its concentration,and inhibitory rates were higher than that of blank group at 24,48,72 h. Flow cytometry results showed the apoptosis rate of novel ruthenium coordination compound groups of 10,30,50 μmol / L were( 17. 64 ± 1. 21) %,( 26. 47 ±0. 61) %,( 55. 63 ± 1. 49) %,respectively,all higher than that of blank group( P < 0. 05). The cell proportion of G1 phase increased with the increasing of the novel schiff base ruthenium coordination. Conclusion A novel schiff base ruthenium coordination compound could inhibit the growth of gastric cancer SGC-7901 cells,promote apoptosis and arrest gastric cancer SGC-7901 cells at G1.

关 键 词：新型希夫碱钌配合物 胃癌SGC-7901细胞 细胞增殖 细胞凋亡 细胞周期 

分 类 号：R730.5[医药卫生—肿瘤]