单质粒模式诱导表达载体系统的建立  

Design and Construction of Inducible Expression Vector by Single-Plasmid Strategy

作　　者：刘定燮[1] 周晓巍[1] 黄培堂[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《生物化学与生物物理进展》2003年第5期824-825,共2页Progress In Biochemistry and Biophysics

基　　金：中国博士后科研基金资助项目~~

摘　　要：在Invitrogen公司T Rex诱导表达系统的基础上,将目的基因与调控蛋白基因克隆于同一载体上,并在CV1细胞中观察了四环素对该单质粒模式载体报告基因表达的诱导效应.载体在瞬时转染CV1细胞并以四环素诱导 2 4h后目的基因的表达水平提高了 8 9倍。qOn the basis of T-Rex inducible expression system, a vector that express reporter gene and regulated gene simultaneously were constructed. The induction effects of tetracycline to the gene expression were studied in CV1 cells by transient transfection. An 8.9-fold increase in expression level of reporter gene was observed when CV1 cells, which were transiently transfected with the vector, were induced with tetracycline for 24 h, suggesting that the application of single-plasmid vecor in gene expression induction is also feasible.

关 键 词：单质粒模式 诱导表达 载体系统 四环素 

分 类 号：Q782[生物学—分子生物学]