QuEChERS-气相色谱分析高原夏菜中有机磷农药残留  

Determination of organophosphorus pesticide residues in plateau summer vegetables by QuEChERS-gas chromatography

作　　者：叶永丽[1] 赫欣睿 陈士恩[1] 高旭东[1] 马忠仁[1,2] 

机构地区：[1]西北民族大学生命科学与工程学院,兰州730030 [2]西北民族大学甘肃省动物细胞工程技术研究中心,甘肃兰州730030

出　　处：《食品安全质量检测学报》2015年第6期2164-2170,共7页Journal of Food Safety and Quality

基　　金：甘肃省农牧厅现代农业生产发展资金([2014]349);教育部创新团队发展计划(IRT13091)~~

摘　　要：目的建立同时测定高原夏菜中甲胺磷、敌敌畏、乙酰甲胺磷等12种有机磷农药残留的QuEChERS-气相色谱(quick,easy,cheap,effective,rugged,safe-gas chromatography,QuEChERS-GC)分析方法。方法样品用乙腈进行超声辅助提取,提取液经N-丙基乙二胺(PSA)和石墨化炭黑(GCB)进行分散固相萃取净化,气相色谱-火焰光度检测器(GC-FPD)进行分析测定,外标法定量。结果 12种有机磷农药的检出限范围为3.4~20.8μg/kg,相对标准偏差在2.95%~8.44%之间,平均回收率范围为83.17%~102.62%。结论方法前处理操作简单,检测的重复性好、精密度高,可满足对蔬菜中多种有机磷农药残留的同时分析。ABSTRACT:Objective To establish a gas chromatography method for simultaneously determination of 12 kinds of organophosphorus pesticide residues (OPPs) in plateau summer vegetables. Methods Samples were extracted by acetonitrile assisted with ultrasonic, cleaned up by dispersive solid phase extraction combining with GCB and PSA. The targets were analyzed by GC-FPD using an external standard method. Results The detection limits and the average recoveries were ranged from 3.4 to 20.8 μg/kg, and 83.17% to 102.62%, respectively, the relative standard deviation (RSD) was between 2.95%~8.44%. Conclusion The pretreatment of this method is simple, and has a good repeatability and high precision which can meet the need of OPPs analysis in vegetables.

关 键 词：气相色谱法 高原夏菜 有机磷农药残留 

分 类 号：O657.71[理学—分析化学] TS207.53[理学—化学]