杏鲍菇多糖对S180荷瘤小鼠的保护作用  

Protective Effects of Pleurotus eryngii Polysaccharides on S180 Tumor-Bearing Mice

作　　者：刘海英[1] 范刘士博 

机构地区：[1]唐山市农业科学研究院,河北唐山063000 [2]天津市宝坻区第四中学,天津301800

出　　处：《食品科学》2015年第3期198-201,共4页Food Science

基　　金：河北省自然科学基金项目(10220126)

摘　　要：目的:分析杏鲍菇多糖对S180荷瘤小鼠的保护能力和对化疗效果的影响。方法:将小鼠随机分为6 组:空白对照组(A组)、用糖对照组(B组)、肿瘤模型组(C组)、化疗组(D组)、感染用糖组(E组)和化疗用糖组(F组)。其中"用糖"是指在40 d的实验期间内一直腹腔注射100 mg/(kg·d)(以体质量计,下同)杏鲍菇多糖,"感染"是指在实验第1天一次性后肢皮下注射3 mL/kg新鲜制备的含有S180肿瘤细胞的种鼠腹水;"化疗"是指小鼠接种S180细胞10 d后腹腔注射5.0 mg/kg顺铂,间隔1 d注射一次,共注射5 次。接种20～40 d时观测死亡率、体质量、白细胞数量和腹水超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活性及丙二醛(malondialdehyde,MDA)浓度。结果:杏鲍菇多糖能够显著降低S180荷瘤小鼠的死亡率,延长小鼠存活时间,增加血液内白细胞数量,增强腹水SOD和CAT的酶活性,降低MDA浓度,降低化疗对小鼠免疫能力的影响(P<0.05)。结论:杏鲍菇多糖对S180荷瘤小鼠具有极强的保护作用,并且能够降低顺铂化疗的副作用。Objective: This study aimed to examine the protective effects of Pleurotus eryngii polysaccharides(PePs) on mice bearing sarcoma 180(S180) cells and the efficacy of PePs in reducing the side-effects of chemotherapy with cisplatin.Methods: Healthy male mice were randomly divided into blank control group(A), PePs treatment group(B), model blank group(C), chemotherapy group(D), Peps treatment group(E) and Pep treatment plus chemotherapy group(F). PePs were intraperitoneally injected at a dose of 100 mg/(kg·d) body weight(bw) daily for 40 days. A single dose of ascites fluid(3 mL/kg bw) from mice inoculated with S180 was subcutaneously injected on the first day of experiment. Chemotherapy was achieved by five repeated intraperitoneal injections of 5.0 mg/kg bw of cisplatin every other day from 10 days after administration of S180 cells. During 20–40 days after inoculation of S180 cells, the death rate, body weight, blood leucocyte number, superoxide dismutase(SOD) and catalase(CAT) activities, malondialdehyde(MDA) concentration in the ascites fluid of mice were measured. Results: The fungal polysaccharides significantly reduced the mortality rate of S180-bearingmice, prolonged the survival time, increased the number of blood leucocytes, enhanced the activities of ascites SOD and CAT enzymes, decreased the ascites MDA concentration, and reduced the adverse effects of chemotherapy on mouse immunity(P < 0.05). Conclusion: PePs possess strong protective effects on S180-bearing mice and reduce the side effects of cisplatin chemotherapy.

关 键 词：杏鲍菇多糖 S180肿瘤细胞 化疗 保护作用 

分 类 号：R965[医药卫生—药理学]