NKA-Ⅱ树脂富集纯化银杏叶聚戊烯醇  

Enrichment and Purification of Ginkgo biloba Leaf Polyprenols by NKA-Ⅱ Macroporous Resin

作　　者：刘玉桃 杨小明[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《食品科学》2015年第4期63-67,共5页Food Science

基　　金：镇江市工业攻关项目(GY2011015)

摘　　要：从8种树脂中筛选出对银杏叶聚戊烯醇(polyprenols,PPs)有较大吸附率的NKA-Ⅱ树脂。研究PPs在NKA-Ⅱ树脂上的吸附特性,确定分离PPs适宜工艺参数。动力学数据研究表明,PPs在NKA-Ⅱ树脂上的吸附过程符合一级吸附动力学方程,控速步骤以液膜扩散为主;吸附等温曲线服从Langmuir和Freundlich等温吸附方程。NKA-Ⅱ树脂固定床纯化PPs的操作条件:上样质量浓度0.73 mg/m L、流速2 m L/min;以流速1 m L/min的正己烷-丙酮(80∶20,V/V)洗脱,用量5 BV。经NKA-Ⅱ树脂固定床纯化后,PPs含量从33.85%提高到71.54%,回收率为88.76%。树脂柱再生第6次时,对PPs的分离纯化能力才明显降低,表现出良好的再生使用性。Resin NKA-Ⅱ was selected as the best one among eight kinds of macroporous resins for the purification of polyprenols(PPs) from Ginkgo biloba leaves in terms of its highest adsorption rate for PPs. The adsorption behavior of PPs on NKA-Ⅱ resin was further investigated and optimum parameters for adsorption and desorption were procured. The data showed that the adsorption behavior of PPs on NKA-Ⅱ resin followed the first order kinetic equation. The adsorption process was mainly affected by film diffusion. The adsorption isothermal curve obeyed both Freundlich and Langmuir isotherm equations. PPs were separated by a fixed bed packed with NKA-Ⅱ resin under the operating conditions as follows: 0.73 mg/m L of the sample was loaded onto the column at a flow rate of 2 m L/min and eluted with 5 BV of hexane-acetone(80:20, V/V) at a flow rate of 1 m L/min. Under these conditions, the content of PPs increased from 33.85% to 71.54%, and the recovery was 88.76%. After the sixth regeneration, its separation capacity for PPs began to significantly decrease, showing a good regenerability.

关 键 词：大孔树脂 吸附 聚戊烯醇 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]