转染IL-18基因的大鼠胶质瘤细胞疫苗9L/IL-18对胶质瘤大鼠中TGF-β和Fgf2水平的影响  

The effect on the expression of TGF- β and Fgf2 of the exogenous IL- 18 gene on the biological characteristics of rat 9L glioma cells

作　　者：程天军[1] 宋杨英[2] 

机构地区：[1]河北省玉田县医院肿瘤外科,河北玉田064100 [2]河北省玉田县医院检验科,河北玉田064100

出　　处：《现代肿瘤医学》2016年第5期713-715,共3页Journal of Modern Oncology

摘　　要：目的:观察转染IL-18基因的大鼠胶质瘤细胞疫苗9L/IL-18对胶质瘤大鼠血清TGF-β和肿瘤组织中Fgf2水平的影响。方法:将F344大鼠随机分为3组,分别接种9L/IL-18、9L/LXSN和9L细胞。ELISA法检测大鼠血清中TGF-β水平,RT-PCR方法检测肿瘤组织中Fgf2 mRNA的表达。结果:接种9L/IL-18细胞大鼠的外周血中TGF-β的水平低于其他两组,差异有统计学意义(P<0.05),肿瘤组织中Fgf2 mRNA的表达均低于其他两组(P<0.05)。结论:IL-18通过降低TGF-β和Fgf2的水平,起到抗肿瘤的作用。Objective: To assess the effect on the expression of TGF- β and Fgf2 of the exogenous IL- 18 gene on the biological characteristics of rat 9L glioma cells. Methods: The cell suspension of 9L / IL- 18,9L / LXSN and 9L cells were inoculated into the brain of F344 rat,detct the level of TGF- β and Fgf2 by enzymelinked immunosorbent assay and RT- PCR. Results: The level of TGF- β in the peripheral blood of rats inoculated 9L / IL- 18 was weakend to that inoculated 9L / LXSN and 9L( P < 0. 05). Compared to 9L / LXSN and 9L cells inoculated rat,the expression of Fgf2 were decreased in the tumor tissue of rat inoculated 9L / IL- 18. Conclusion: IL- 18 could decrease the level of TGF- β and Fgf2.

关 键 词：IL-18 胶质瘤 抗肿瘤 血管生成 

分 类 号：R739.41[医药卫生—肿瘤]