拉布拉多犬神经类型相关基因SNP的分析  

Analysis of SNPs Associated with Nerve Pattern Related Genes in Labrador Retrievers

作　　者：赵明媛[1] 俞剑熊 韩芳[1] 李雅婵[1] 张雅丽[1] 周子娟[1] 王福金[1] 王爱国[1] 王靖宇[1] 

机构地区：[1]大连医科大学实验动物中心,大连116044

出　　处：《实验动物科学》2015年第3期38-43,共6页Laboratory Animal Science

基　　金：国家自然科学基金(No:31272392)

摘　　要：目的检测拉布拉多犬基因组DNA中与神经类型密切相关的4个基因中的6个SNP位点,以期为拉布拉多犬神经类型的早期鉴定提供分子遗传学鉴定指标。方法依据中国导盲犬大连培训基地成熟的导盲犬神经类型行为学评估标准,选取典型的安静、活泼、胆小型(弱型)三种神经类型的犬各4只为研究对象,采取静脉血,提取血细胞的基因组DNA样本,采用PCR/测序方法和PCR-RFLP分析法对12个样本4个基因的6个SNP位点:COMT(G482A)、MOAB(T199C)、ABCB1(A985T、T3442C、377-378 ins C)、GNB1L(961-962 ins G)进行变异型检测,并统计分析SNP位点的变异型与神经类型的关联度。结果 COMT基因G482A SNP位点的G/A型在胆小型犬中所占比例显著高于其在活泼型和安静型中所占比例(P=0.014;P=0.014),A/A型在活泼型犬中所占比例显著高于安静型和胆小型中所占比例(P=0.025;P=0.025);MAOB基因T199C SNP位点的T/C型在安静型犬中所占比例显著高于其在活泼和胆小型犬中所占比例(P=0.025;P=0.025);ABCB1基因A985T SNP位点的T/A型在活泼型犬中所占比例显著低于其在安静和胆小犬中所占比例(P=0.014;P=0.014);ABCB1基因T3442C SNP位点的T/C型在胆小型犬中所占比例显著高于其在活泼和安静型犬中所占比例(P=0.025;P=0.025);ABCB1基因的377-378 ins C和GNB1L基因的961-962 ins G均没有发现显著性差异(P>0.05)。结论 COMT基因G482A SNP位点的G/A型和A/A型,MAOB基因T199C SNP位点的T/C型,ABCB1基因A985T SNP位点的T/A和ABCB1基因T3442C SNP位点的T/C型与拉布拉多犬神经类型具有显著相关性,可做为拉布拉多犬神经类型早期鉴定的分子遗传诊断指标,但尚需大样本确认。Objective Analysis of 6 SNPs associated to nerve pattern related 4 genes in Labrador Retrievers to provide a early-stage molecular genetic index for nerve pattern diagnostics. Method According to the behavior evaluation standards of China guide dog training center,the dogs belong to typical nerve patterns( quiet,active,and timid( weak) type,4 dogs each) were selected. The vein blood was sampled and the genomic DNA was isolated from blood cells. The PCR / sequencing and PCR-RFLP method were used to analyze the variants of 6 SNPs in nerve pattern related 4 genes:( COMT( G482A),MOAB( T199C),ABCB1( A985 T,T3442C,377-378 ins C),GNB1L( 961-962 ins G)). Furthmore,the association between the SNP variants and nerve patterns was analyzed by Chi-square Test. Result G / A variants in COMT( G482A) SNP locus in the proportion of timid dogs was significantly higher than that in the active and quiet dogs( P = 0. 014; P = 0. 014),A / A variants in COMT( G482A) SNP locus in the proportion of active dogs was significantly higher than that in the quiet and timid dogs( P = 0. 025; P = 0. 025); T/C variants in MAOB( T199C) SNP locus in the proportion of quiet dogs was significantly higher than that in the active and timid dogs( P = 0. 025; P = 0. 025); T / A variants in ABCB1( A985T) SNP locus in the proportion of active dogs was significantly lower than that in the quiet and timid dogs( P= 0. 014; P = 0. 014); T / C variants in ABCB1( T3442C) SNP locus in the proportion of timid dogs was significantly lower than that in the quiet and active dogs( P = 0. 025; P = 0. 025); No ABCB1( 377-378 ins C)and GNB1L( 961-962 ins G) SNP loci was found. Conclusion G / A and A / A variants in COMT( G482A) 、T / C variants in MAOB( T199C) 、T / A variants in ABCB1( A985T) and T / C variants in ABCB1( T3442C) are significantly associated to nerve patterns and might be used as a molecular genetic index for nerve pattern diagnostics in Labrador Retrievers. However,further large sample analysis is required.

关 键 词：拉布拉多犬 神经类型 单核苷酸多态性(SNP) 分子遗传学诊断 

分 类 号：S829.2[农业科学—畜牧学]