常规饲料添加蛋黄和花生米的方法建立大鼠肥胖模型  

Construction of Obesity in Rats with Normal Diet Adding Egg Yolk and Peanuts

作　　者：李鸿雁[1] 颜克松[1] 战大伟[1] 

机构地区：[1]解放军总医院第一附属医院,北京100037

出　　处：《实验动物科学》2015年第3期48-50,61,共4页Laboratory Animal Science

基　　金：国家自然科学基金(NO.31201323)

摘　　要：目的通过常规饲料添加蛋黄和花生米的方法诱发建立雄性SD营养性肥胖大鼠动物模型。方法 30只4周龄SD雄性大鼠随机分为实验组24只,对照组6只。实验组饲喂正常饲料并添加鸡蛋黄及花生米,饲喂2周后实验组剔除肥胖抵抗大鼠,对照组饲喂正常饲料。大鼠饲喂8周后称量大鼠体质量并计算Lee's指数,禁食12 h后处死,腹主动脉取血分离血清,检测血清中总胆固醇(TC)、甘油三脂(TG)含量,同时取肝脏组织进行组织病理学检查。结果实验组大鼠饲喂8周后,体质量达到526.6±46.4 g,对照组大鼠体质量为415.6±33.7 g,实验组大鼠体质量超过对照组体质量26.7%;实验组的Lee's指数为330.0±8.2,对照组Lee's指数为307.0±6.6(P<0.01),实验组与对照组比较有极显著差异。实验组的大鼠的血清TG均值为2.16±0.17 mmol/L、TC均值为1.40±0.34 mmol/L,对照组血清TG均值为1.66±0.11 mmol/L,TC均值为0.82±0.13 mmol/L,两组大鼠比较有极显著性差异。病理学结果显示:实验组大鼠肝脏均出现脂肪样变。结论 4周龄SD雄性大鼠采用正常饲料并添加鸡蛋黄及花生米的方法能稳定成功地建立营养性肥胖大鼠模型。Objective To establish high fat diet induced obesity in male SD rat model of nutrition. Method Four weeks old male SD rats were randomly divided into experimental group( n = 24) and control group( n = 6). The control group was fed normal diet and the experimental group was fed high fat diet which was composed with normal diet,egg yolk and peanuts. Two weeks later,the obesity resistant rats were excluded from the experimental group.Weight and Lee’s index were observed after eight weeks. After twelve hours fasted,all rats were sacrificed and tested for TC、TG. The hepatic tissue of rats was analyzed histomorphologically. Result Experimental group weighted 526. 6 ± 46. 4g and the control group weighted 415. 6 ± 33. 7g,there for the weight of experimental group rats were 33. 1% higher than control group rats. Lee’s index of the experimental group was 330. 0 ± 8. 2,while the control group was 307. 0 ± 6. 6. The serum total cholesterol of experimental group was 2. 16 ± 0. 17 mmol / L,serum triglyceride was 1. 40 ± 0. 34 mmol / L and control group,s serum total cholesterol was 1. 66 ± 0. 17 mmol / L,serum triglyceride was 0. 82 ± 0. 13 mmol / L. Pathology results showed the experimental group were induced fatty liver.Conclusion This study showed that feeding high fat diet which was composed with normal diet,egg yolk and peanuts could successfully and stably established obesity model.

关 键 词：高脂饲料 饲喂方式 SD大鼠 Lee’s指数 

分 类 号：R[医药卫生] R589.2