绿色荧光裸鼠部分免疫学指标的测定及分析  

Measurement of Some Immunological Parameters in GFP Transgenic Nude Mice

作　　者：尤金炜[1] 刘彪[1] 张旭亮[1] 马畅[1] 董敏[1] 赵志刚[1] 恽时锋[1] 

机构地区：[1]南京军区南京总医院比较医学科全军实验动物科普与伦理教育基地全国科普教育基地,南京210002

出　　处：《实验动物科学》2015年第5期15-17,21,共4页Laboratory Animal Science

基　　金：南京军区南京总医院院管课题(No.2013002)

摘　　要：目的检测绿色荧光蛋白(Green Fluorescence Protein,简称GFP)转基因裸鼠的部分免疫学指标,分析GFP转基因裸鼠与其他小鼠在免疫学方面的优缺点,为将来的研究提供基础参考值。方法 1实验选用6~8周GFP转基因裸鼠、BALB/c裸鼠和BALB/c小鼠,活体称体质量,剖取脾脏称其质量,之后进行脾淋巴细胞增殖能力的测定。2取外周血,测定部分血常规指标及免疫球蛋白指标。结果 1 GFP转基因裸鼠脾脏质量、脾脏系数以及T、B淋巴细胞增殖反应与BALB/c裸鼠相近,但与BALB/c小鼠差异显著。2 GFP转基因裸鼠、BALB/c裸鼠两种裸鼠品系外周血白细胞水平、淋巴细胞以比例及免疫球蛋白IgG、IgM都远低于BALB/c小鼠,差异显著。结论从脏器系数、白细胞水平,免疫球蛋白水平等角度发现,GFP转基因裸鼠和BALB/c裸鼠的免疫系统水平较BALB/c小鼠相弱,其中GFP转基因裸鼠的免疫系统水平最为低下。Objective To measurement some immunological parameters of transgenic nude mice with Green Fluorescent Protein. Analyze the advantages and disadvantages of GFP transgenic nude mice and other mice. To provide basis for future research. Method 16 ~ 8 weeks mice in each,were used in this experiment. Live weigh,anatomy and take the spleen weighing. The proliferation ability of spleen lymphocyte was determined. 2 Blood samples were measured using an automatic analyzer and ELISA. Result 1 The body weight,spleen index,T and B lymphocyte proliferative response of GFP transgenic nude mice were similar to BALB / c nude mice. But there was a significant difference between the two and BALB / c mice. 2 The levels of leukocytes,lymphocytes in the peripheral blood of two nude mice,and the proportion of IgG and IgM were much lower than that of BALB / c mice. Conclusion The immune system of GFP transgenic nude mice and BALB / c nude mice were weaker than that of BALB / c mice,and the level of immune system in GFP transgenic mice was the lowest.

关 键 词：GFP转基因裸鼠 BALB/C裸鼠 BALB/C小鼠 免疫功能 

分 类 号：R-33[医药卫生]