H_2O_2预处理对PC12细胞氧化应激损伤的适应性细胞保护作用  

The adaptive cytoprotection of H_2O_2 preconditioning against oxidative-stress damage in PC12 cell

作　　者：唐小卿[1] 冯鉴强[1] 李平阳[1] 郭瑞鲜[1] 叶美红[1] 

机构地区：[1]中山大学中山医学院生理学教研室,广州518007

出　　处：《中国药理学通报》2003年第10期1115-1118,共4页Chinese Pharmacological Bulletin

摘　　要：目的　探讨H2 O2 预处理对PC12细胞氧化应激损伤的适应性细胞保护作用及与脑源性神经营养因子 (brain de rivedneurotrophicfactor,BDNF)的关系。 方法　采用MTT法检测细胞活力 ,PI染色流式细胞术检测细胞凋亡 ,间接免疫荧光流式细胞术检测细胞BDNF的表达。结果　PC12细胞经 10 μmol·L-1H2 O2 预处理 90min后 ,2 0～ 6 0 μmol·L-1H2 O2 对PC12细胞生长的抑制程度明显下降 ,H2 O2 (2 0、30μmol·L-1)对PC12细胞凋亡的诱导作用明显受到抑制 ,BD NF的表达强度增强。结论　H2 O2 预处理对PC12细胞氧化应激损伤具有保护作用 。AIM To study the adaptive cytoprotection of H 2O 2 preconditioning against oxidative stress damage in PC12 cell and the relationships between brain derived neurotrophic factor (BDNF) and the adaptive cytoprotection of H 2O 2 preconditioning. METHODS The viability of PC12 cells was evaluated by MTT assay. Apoptosis was detected by PI stain flow cytometer. Expression of BDNF was analyzed by immuo flow cytometer. RESULTS After H 2O 2 preconditioning, the survival of PC12 cells exposure to H 2O 2 (20～60 μmol·L -1 ) was increased and the apoptosis of PC12 cells induced by H 2O 2 (20 or 30 μmol·L -1 ) was inhibited and the expression of BDNF in PC12 cells was enhanced. CONCLUSION H 2O 2 preconditioning is protective against the damage of PC12 cells induced by H 2O 2 and its mechanisms may involve up modulation of the expression of BDNF.

关 键 词：H2O2 预处理 氧化应激损伤 适应性细胞保护 脑源性神经营养因子 

分 类 号：R-332[医药卫生] R329.24