氟哌啶醇季铵盐衍生物对大鼠心室肌细胞L型钙电流的影响  

Effect of quaternary ammonium salt derivative of haloperidol on L-type currents in rat ventricular myocytes

作　　者：黄展勤[1] 石刚刚[1] 郑锦鸿[1] 高分飞[1] 刘幸平[1] 

机构地区：[1]汕头大学医学院药物研究室,汕头515031

出　　处：《中国药理学通报》2003年第10期1129-1131,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助;No 3 0 0 70 3 0 4;广东省科技计划项目资助 ;NoC3010 4;广东省自然科学基金重点项目资助;No 62 12 3 5

摘　　要：目的　研究氟哌啶醇季铵盐衍生物 (F2 )对大鼠心室肌细胞L型钙电流 (ICa)的影响。方法　采用酶急性分离的单个大鼠心室肌细胞 ,应用膜片钳全细胞记录技术 ,观察F2对ICa的影响。结果　F2 (1μmol·L-1)能抑制任何一膜电压下的ICa,使峰电流从 (1775 2± 36 0 4 ) pA减少至 (46 4±12 9 1) pA (n =8,P <0 0 1) ,并使ICa的电流 -电压曲线上移 ,但不改变ICa的电压依赖特征。结论　F2AIM To study the effect of quaternary ammonium salt derivative of haloperidol (F 2) on L type calcium current ( I Ca ) in rat ventricular myocytes. METHODS Single ventricular cell of rat was obtained by enzymatic dissociation method. The currents were recorded with the whole cell configuration of the patch clamp technique. RESULTS F 2(1 μmol·L -1 ) decreased I Ca from ( 1 775 2 ±360 4) pA to (464±129 1) pA ( n =8, P< 0 01) and shifted the current voltage curve of I Ca upward, without affecting the voltage dependent properties of I Ca . CONCLUSION F 2 inhibited L type calcium current in rat ventricular myocytes.

关 键 词：氟哌啶醇 心肌细胞 钙通道 膜片钳 

分 类 号：R-332[医药卫生] R322.11