K-ras基因转染鼠肺纤维细胞粘附分子的表达及临床意义  

Expression and Clinical Significance of Adhesive Molecule in Lung Fibrocyte of K-ras Transformed Rat

作　　者：张其刚[1] 宿杰[2] 尚文军[1] 关永信 

机构地区：[1]中国医科大学附属第一医院胸外科,辽宁沈阳110001 [2]沈阳市第七人民医院胸外科 [3]营口市经济技术开发区人民医院

出　　处：《中国医科大学学报》2003年第5期394-395,441,共3页Journal of China Medical University

摘　　要：目的 :探讨细胞粘附分子在肿瘤细胞浸润转移中的意义。方法 :利用免疫荧光标记及聚丙烯酰胺凝胶电泳和Westernblot观察K ras基因转染的鼠肺 (KTRL)纤维细胞及正常鼠肺 (NRL)纤维细胞钙粘素 链蛋白复合体分子的表达。结果 :KTRL纤维细胞粘附分子表达明显异常 ,肌蛋白分布方式发生改变 ,粘附能力明显下降。结论 :KTRL纤维细胞作为肿瘤细胞的早期阶段 ,其粘附能力的改变 。Objective: Our aim was to study the function of adhesive molecule in the process of tumor cell invasion. Methods: We studied lung fibrocytes in K-ras transformed rats and normal rats. The expression of cadherin-catenin complex was observed with immunofluorescence labeling technique, polyacrylamide gel electrophoresis, and western blot. Results: Obviously abnormalities was found in the expression of adhesive molecule of lung fibrocytes in K-ras transformed rats. The distribution of actin changed with significance decrease of adhesion. Conclusion: The change of cell adhesion in early stage of tumor may be a major factor for cell invasion and metastasis in advanced stage.

关 键 词：K-ras转染鼠肺纤维细胞 粘附分子 浸润 转移 

分 类 号：R730.21[医药卫生—肿瘤]