原位杂交法检测老年急性白血病多药耐药基因表达  

Detection of Mdr1 gene expression in acute leukemias of old ages in situ hybridization with Photoallergy-havitol-labeled RNA probe

作　　者：王春燕[1] 姜振宇[1] 姚程[1] 李舜华[1] 袁长吉[1] 张福明[1] 

机构地区：[1]吉林大学第一医院血液科,吉林长春130021

出　　处：《中国老年学杂志》2003年第8期489-491,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助 (391 70 366)

摘　　要：目的　将简便可靠的非同位素原位杂交方法应用于老年急性白血病多药耐药基因 (mdr1 )的临床检测。方法　经光敏生物素标记的RNA探针 ,直接在细胞甩片上进行原位杂交 ,检测 40例老年急性白血病骨髓细胞 mdr1 m RNA。结果　杂交信号清晰 ,重现性好。难治复发病人中mdr1阳性率 77.2 7% ,初治病人 33.33% ,差异有显著性。结论　光敏生物素标记的 RNA探针原位杂交方法简便、快速、安全、可靠 ,可在临床用于老年急性白血病 mdr1常规检测 ,辅助临床治疗方案设计及判断预后。Objective To detect the mdr1 gene in acute leukemia of old ages.Methods In situ hybridization(ISH) were performed with Photoallergy havitol labeled RNA probes.Results The positive rate of mdr1 expression in relapse and refractory patients was much more higher than that in initial cases( P <0.001).Conclusions The ISH method could be used satisfactorily in predicating clinical drug resistance and directing the rearrangement of chemotherapy regimens in view of its sensitivity and simple procedure.

关 键 词：原位杂交法 老年人 急性白血病 多药耐药基因 基因表达 光敏生物素 

分 类 号：R733.7[医药卫生—肿瘤] R730.53[医药卫生—临床医学]