多发性硬化患者脑脊液和血清中髓鞘碱蛋白和白介素-16的相关研究  

A correlative study of MBP and IL-16 in cerebrospinal fluid and serum of patients with multiple sclerosis

作　　者：赵薛旭[1] 田作军[1] 李作汉[1] 张帆[1] 

机构地区：[1]南京医科大学附属脑科医院,210029

出　　处：《临床神经病学杂志》2003年第4期205-207,共3页Journal of Clinical Neurology

摘　　要：目的　了解多发性硬化 (MS)患者脑脊液 (CSF)和血清髓鞘碱性蛋白 (MBP)和白介素 16 (IL 16 )的水平 ,探讨两者之间的相关性 ,及其在MS发病过程中的作用。方法　采用ELISA法对 31例MS患者CSF和血清的MBP和IL 16进行测定 ,并与 2 4例炎性脱髓鞘性多发性神经病 (IDP)、2 2例对照者进行比较。结果　MS组CSF和血清中的MBP水平均显著高于IDP组及对照组 (均P <0 .0 1) ;CSF中的IL 16的水平显著高于对照组 (P <0 .0 1) ,而血清中IL 16水平与对照组相比无明显差异 (P >0 .0 5 )。MS组中CSF的MBP水平与IL 16水平显著相关 (r=0 .4 6 8,P <0 .0 1) ,但血清中MBP的水平与IL 16水平无相关性 (r=- 0 .131,P >0 0 5 ) ;CSF中MBP水平和血清中MBP的水平有相关性 (r=0 .5 0 5 ,P <0 .0 1) ,CSF中IL 16和血清中IL 16的水平无相关性 (r=0 .0 2 2 ,P >0 .0 5 )。结论　MBP是诱导MS发病的主要自身抗原之一 ,MBP可能主要在中枢神经系统中刺激IL 16的产生 ;而ILObjective To detect the levels of MBP and IL 16 in cerebrospinal fluid(CSF) and serum, investigate the correlation between them and the role in multiple sclerosis attacking process.Methods The levels of MBP and IL 16 of cerebral fluid and serum of 31 patients with MS were detected by ELISA and compared with those of 24 cases of inflammatory demyelinating polyneuropathy or 22 cases of healthy persons.Results CSF and serum levels of MBP were found increaed significantly in MS patients compared with those in IDP and in healthy controls(HC)(all P <0.01) while CSF level of IL 16 increased significantly in MS patients compared with that in HC( P <0.01 ),but no difference was found in the serum level of IL 16 between MS and HC( P >0.05).The concentrations of MBP in CSF of MS significantly correlated with that of IL 16 in CSF( r =0.468, P <0.05),but no correlations were found between MBP and IL 16 in serum( r =-0.131, P >0.05).The concentration of MBP in CSF of MS significantly correlated with CSF that in serum( r =0.505, P <0.01).The level of IL 16 in serum showed no relation with that in CSF( r =0.022, P >0.05).Conclusion MBP is one of the autoantigens that induce MS,IL 16 may be mainly produced in CNS due to MBP stimulation; IL 16 may act within CNS.

关 键 词：多发性硬化 脑脊液 血清 髓鞘碱蛋白 白介素-16 ELISA 中枢神经系统 

分 类 号：R744.51[医药卫生—神经病学与精神病学]