诱导分化剂和热应激对K562细胞JWA和热应激蛋白70表达的影响  

Effect of differentiation inducer and heat stress on the expression of JWA protein and Hsp70 of K562 cells

作　　者：茆文革[1] 李爱萍[1] 叶健[1] 黄曙[1] 李爱群[1] 周建伟[1] 

机构地区：[1]南京医科大学公共卫生学院分子毒理室,210029

出　　处：《中华劳动卫生职业病杂志》2003年第4期253-256,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目 (3 0 0 70 664 ;3 0 170 812 ) ;江苏省高校高新技术产业项目 (JH0 1 49) ;国家重点基础研究发展规划项目(2 0 0 2CB5 12 90 0 )

摘　　要：目的　研究诱导K5 6 2细胞分化和热应激过程中 ,JWA表达的特点 ,探讨JWA与热应激蛋白 (Hsp70 )表达的关系以及参与诱导分化和热应激可能的机制。方法　分别建立K5 6 2细胞诱导分化和热应激模型 ,采用Western blot方法检测JWA、Hsp70、热休克转录因子 (HSF1)、HSF2蛋白表达水平。结果　 (1)佛波酯 (TPA ,10 0ng ml)、氯化高铁血红素 (hemin ,3× 10 - 5mol L)、阿糖胞苷 (Ara C ,80ng ml)、阿霉素 (adriamycin ,4× 10 - 8mol L)、全反式维甲酸 (ATRA ,1× 10 - 6 mol L)、三氧化二砷 (As2 O3,1× 10 - 6 mol L)分别诱导K5 6 2细胞 4 8h ,JWA、Hsp70蛋白表达水平均明显增加 ;HSF2在hemin、Ara C、ad riamycin诱导下表达增加 ;(2 )在 4 2℃不同时间 (10、2 0、30、4 5、6 0、90min)和不同温度 (39℃、4 2℃、4 5℃ )处理下 ,Hsp70表达水平的变化与JWA蛋白表达水平的变化趋势基本相似 ,且HSF1在热应激早期表达。结论　在诱导分化、热应激过程中 ,JWA与Hsp70蛋白表达水平均明显上调 ,且变化趋势有一定的相似 ,但所涉及的细胞内信号转导通路可能不是惟一的。JWA表达与HSF1或HSF2似乎没有直接的特征性联系。Objective To study the expression of JWA protein and heat shock protein(Hsp70),and to explore these relationship and the possible mechanism of JWA gene involved in induced differentiation and heat stress(42 ℃) of K562 cells. Methods The models of differentiation and heat stress of K562 cells were established.Western blot was used for detecting expressed proteins of JWA gene,Hsp70,heat shock factor(HSF1 and HSF2). Results (1)Under the condition of differentiations induced by TPA(100 ng/ml),hemin(3×10 -5 mol/L),Ara-C(80 ng/ml),adriamycin(4×10 -8 mol/L),ATRA(1×10 -6 mol/L) and As 2O 3(1×10 -6 mol/L) for 48 h respectively,the expression of JWA protein and Hsp70 were more significantly increased than control;the level of HSF2 protein was increased by inductions of hemin,Ara-C and adriamycin,respectively.(2)After heat exposure to 42 ℃ for 10,20,30,45,60,90 min, and heat exposure to 39 ℃,42 ℃,45 ℃,the trend of changing in expression of Hsp70 was similar to that of JWA protein,and HSF1 was expressed in earlier stage. Conclusion The expression of JWA protein and Hsp70 were upregulated in induced differentiation and in heat stress,and the change of expression of JWA protein were similar to that of Hsp70,but the intracellular transduction signal pathways involved may be various.JWA might not be specifically related with both HSF1 and HSF2.

关 键 词：白血病 K562细胞 JWA基因 热应激蛋白70 诱导分化剂 热应激 

分 类 号：R73-3[医药卫生—肿瘤]