瘢痕疙瘩p53基因突变高发区基因结构的研究  

Investigation of p53 gene mutations in keloids using PCR-SSCP

作　　者：刘永波[1] 高建华[1] 段红杰[1] 刘晓军[1] 

机构地区：[1]第一军医大学南方医院整形外科

出　　处：《中华整形外科杂志》2003年第4期258-260,共3页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金资助项目 ( 3 9870 80 7)

摘　　要：目的　探讨p5 3抑癌基因在病理性瘢痕组织中的结构异常与临床病理的关系。方法　采用PCR SSCP及基因测序技术 ,以增生性瘢痕及正常皮肤为对照 ,检测瘢痕疙瘩组织标本p5 3基因突变高发区外显子 4～ 6的基因结构。结果　瘢痕疙瘩p5 3基因外显子 4、5、6均存在突变 ,对照组未发现突变。结论　提示瘢痕疙瘩p5 3基因抑制细胞进程及介导细胞凋亡等功能丧失。Objective To detect gene mutations of p53 gene (exon 4～6)in fibroblasts. Methods Samples of keloids were taken from 15 patients. The mutations of p53 gene were detected using polymerase chain reaction, the single-strand conformational polymorphism(SSCP) analysis and DNA sequencing. Results Gene mutations in p53 gene exon 4, 5, and 6 were identified in all the patients with keloids. Conclusion Gene mutations resulted in keloid p53 protein losing its functions of suppressing cell processes and conducting apoptosis. ;

关 键 词：瘢痕疙瘩 P53基因 基因突变 PCR-SSCP技术 基因测序技术 

分 类 号：R622[医药卫生—整形外科]