云南省景颇族人群HIV-1感染相关基因多态性研究  

Polymoprphism of HIV -1 Resistance Genes in Jingpo Ethnic Group of Yunnan

作　　者：彭林 王福生[2] 邱雅 金磊[2] 丁明 刘明旭[2] 叶峻杰 

机构地区：[1]云南省计划生育科学技术研究所,昆明650031 [2]解放军第三零二医院生物工程研究室

出　　处：《中国计划生育学杂志》2003年第8期472-475,共4页Chinese Journal of Family Planning

摘　　要：目的:了解云南省景颇族人群与HIV-1感染相关基因CCR5-Δ32、CCR2-64I、SDF1-3′A的频率和多态性分布。方法:以云南省113例景颇族人群为研究对象,采用PCR、PCB/RFLP(聚合酶链式反应/限制性片段长度多态性分析)计算基因频率,并用统计学方法进行分析。结果:未发现CCR5-Δ32突变;CCR2-64I突变基因频率为16.37％;SDF1-3′G/3′G突变基因频率为17.70％,突变基因频率低于汉族。结论:由于未发现CCR5-Δ32突变和SDF1-3′G/3′G等位基因频率较低,云南省景颇族人群可能比汉族人对HIV-1(包括R5和X4HIV-1)有较大的遗传易感性。Objective: To investigate mutant frequency and polymorphism of HIV - 1 resistance CCR5 -Δ 32, CCR2 - 64I , SDF1 - 3 A alleles in jingf i ethnic group of Yunnan. Methods: The study population included 113 subjectives. The genetypes were respectively detected by polymerase chain reaction(PCR) and by PCRRFLP( restriction fragment length polymorphism) assay. Mutant frequencies were calculated and al-lelic polymorphism in population of the three genes were analyzed by of test. Results: we didnt find CCR5 -A32; the frequency of CCR2 -641 was 16.37% ;and the frequency of SDF1 -3G/3G was 17.70% , lower than Han ethnic group. Conclusion; Compared with Han ethnic group, jingpo ethnic group of Yunnan migtht have a higher genetic susceptibility to HIV - 1 (include R5 and X4 strains) since we didnt find CCR5 -Δ32 and the frequency of SDF1 -3'G/3'G was much lower than that of Han ethnic group.

关 键 词：云南省 景颇族 HIV-1感染 基因多态性 统计学 遗传易感性 协同受体 基因突变 基因频率 人类免疫缺陷病毒 

分 类 号：R394[医药卫生—医学遗传学]