HPLC法测定三七中人参皂苷Rg_1和三七皂苷R_1的含量  

Determination of Ginsenoside Rg1 and Notoginsenoside R1 in Notoginseng by HPLC

作　　者：张雯洁[1] 李忠琼[1] 张赟华[1] 胡旭佳[1] 

机构地区：[1]云南省药品检验所,昆明650011

出　　处：《中国药品标准》2003年第4期35-37,共3页Drug Standards of China

摘　　要：目的:建立HPLC测定三七中人参皂苷Rg_1和三七皂苷R_1含量的方法。方法:色谱条件为:C_(18)柱,流动相为乙腈-0.05％磷酸溶液(21.5:78.5),UV检测波长为203nm。结果:此方法线性关系良好,平均加样回收率分别为人参皂苷Rg_1 99.54％和三七皂苷R_1 101.40％(n=6)。结论:本方法分离良好,结果准确可靠。Objective:To establish the determination method of Ginsenoside Kg1 and Notoginsenoside R1 in Notoginseng. Methods :The HPLC method for determination of Notoginseng was. C18 column (250mm ×4. 6mm, 5um), a mixture acetonitrile-0. 05%phosphoric acid(21.5 : 78. 5)as a mobile phase,detection wavelength 203nm. Results:This method showed a good linear relationship. The mean recovery was Ginsenoside Rg1 99.54% and Notoginsenoside R1 101.40% (n= 6). Conclusion:This method showed a good separating degree and reliable.

关 键 词：HPLC法 含量测定 三七 人参皂苷RG1 三七皂苷R1 

分 类 号：R282.71[医药卫生—中药学]