UVB对HaCaT细胞凋亡的影响  

THE EFFECT OF UVB ON APOPTOSIS OF HaCaT CELLS

作　　者：郭坤[1] 纪凤芝[2] 陶桂兰[2] 陈祥国[3] 武志峰[4] 黄绵庆[5] 王春波[5] 

机构地区：[1]青岛大学医学院实验室管理科,山东青岛266021 [2]青岛大学医学院附属医院资深专家门诊部 [3]中国人民解放军第二炮兵青岛疗养院医务科 [4]青岛大学医学院学生工作办公室 [5]青岛大学医学院药理学教研室

出　　处：《青岛大学医学院学报》2003年第3期299-301,303,共4页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金资助项目 ( 3 9970 63 8)

摘　　要：①目的　探讨紫外线B(UVB)对HaCaT细胞凋亡的影响及其作用机制。②方法　取培养的HaCaT细胞 ,用 30mJ/cm2 的UVB照射后继续培养 18h ,以流式细胞术检测细胞的凋亡率、线粒体膜电位和细胞内游离Ca2 +水平 ,用透射电镜观察细胞的超微结构。③结果　UVB照射增加了HaCaT细胞的凋亡率 (t =5 .2 36 ,P <0 .0 1) ,降低了线粒体膜电位 (t=6 .897,P <0 .0 1) ,升高了细胞内游离Ca2 + 水平 (t =6 .0 4 6 ,P <0 .0 1)。细胞的超微结构因UVB照射而遭到严重破坏 ,胞浆内的细胞器大量减少 ,线粒体严重空泡化 ,髓样小体形成 ,核内染色质成块并边聚。④结论　线粒体膜电位的下降和胞内游离Ca2 +Objective\ To investigate the effect of UVB on apoptosis of HaCaT cells and its mechanism. \ Methods Cultured HaCaT cells were exposed to 30 mJ/cm 2 UVB and subsequently incubated for 18 hours. A flow cytometry was used to measured apoptotic rate, mitochondrial membrane potential and intracellular free Ca 2+ . Ultrastructure of HaCaT cells was observed through transmission electron microscope. \ Results\ UVB irradiation enhanced the apoptotic rate of HaCaT cells ( t= 5.236 , P <0.01), reduced mitochondrial membrane potential ( t=6.897, P <0.01) and elevated the level of intracellular free Ca 2+ ( t=6.064, P <0.01). The cellular ultrastructure underwent severe damage due to UVB irradiation.\ Conclusion\ UVB is able to induced apoptosis of HaCaT cells. Reduction of mitochondrial membrane potential and elevation of intracellular free Ca 2+ are involved in the process.

关 键 词：紫外线B HACAT细胞 细胞凋亡 线粒体膜电位 流式细胞术 

分 类 号：R363[医药卫生—病理学]