大鼠局灶性脑缺血再灌注后脑缺血半影区的界定  

THE DEFINITION OF CEREBRAL ISCHEMI C PENUMBRA AFTER FOCAL CEREBRAL ISCHEMIA REPERFUSION IN RATS

作　　者：陈红兵[1] 王鲁民[1] 陈燕[1] 徐浩文[1] 丰岩清[1] 郭云良[1] 

机构地区：[1]青岛大学医学院脑血管病研究所,山东青岛266003

出　　处：《青岛大学医学院学报》2003年第4期370-371,374,F002,共4页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省自然科学基金资助项目 (编号Y2 0 0 1C0 5 )

摘　　要：①目的　界定大鼠局灶性脑缺血再灌注后脑缺血半影区的范围。②方法　应用线栓法建立大鼠大脑中动脉闭塞再灌注模型 ,氯化三苯基四氮唑 (TTC)染色、甲苯胺蓝染色、焦油紫染色和苏木精 伊红染色界定脑缺血半影区和中心区。③结果　脑缺血再灌注 2h ,缺血病灶位于纹状体外侧区和额顶叶皮质下部 ,随着缺血再灌注时间的延长病灶范围逐渐扩大 ;再灌注 1～ 2d最大 ,波及纹状体内侧区和额顶叶皮质上部以及梨状区皮质 ,之后逐渐缩小 ;再灌注 7～ 14d恢复到再灌流 2h的水平。缺血病灶与周围正常脑组织之间有一形态和结构过渡区 ,该区的着色和细胞形态结构界于病灶和正常脑组织之间 ,即缺血半影区。缺血中心区神经细胞明显减少 ,呈现坏死特征 ;缺血半影区细胞主要呈现核固缩、染色质凝聚等凋亡特征。④结论　脑缺血再灌注缺血中心区位于纹状体外侧区和额顶叶皮质下部 ,缺血半影区位于纹状体内侧区和额顶叶皮质上部以及梨状区皮质。Objective\ To define the histologic range of cerebral ischemic penumbra after focal cerebral ischemia reperfusion in rats.\ Methods\ A model of middle cerebral artery occlusion/reperfusion w as established. The ischemic penumbra and central area were distinguished by TTC , Toluidine blue, Nissl and HE staining methods. \ Results\ The ischemic area located at lower frontoparietal cortex and lateral striatum 2 h after reperfusion, then increased gradually and peaked on 1-2 d and expanded to the upper frontoparietal cortex, piriform cortex and me dial striatum, and returned to reperfusion of 2 h level on day 7-14. There was a transitional area in which the staining color and the structure of neuron wer e between normal and ischemic area. The number of neuron reduced significantly w ith main characteristics of necrosis in ischemic central area and apoptotic prop erty in ischemic penumbra. \ Conclusion\ The ischemic central area located at lower frontoparie tal cortex and lateral striatum, while the ischemic penumbra at upper frontopari etal cortex, medial striatum and piriform cortex.

关 键 词：局灶性脑缺血 再灌注损伤 大鼠 半影区 界定 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]