鲨鱼软骨粉对小鼠移植性肉瘤-180抑制作用的研究  

Study on the inhibition of shark cartilage products on transplanted sarcoma-180 in mice

作　　者：李业鹏 韩春卉 李燕俊 李玉伟 江涛 张靖 计融 

机构地区：[1]中国疾控中心营养与食品安全所,北京100021

出　　处：《中国食品卫生杂志》2003年第5期402-405,共4页Chinese Journal of Food Hygiene

摘　　要：为了观察鲨鱼软骨制品对小鼠移植性肉瘤 - 180 (S 180 )的抑制作用 ,分别经口灌胃给予昆明种小鼠 0 0 2～ 4 5g kgBW 的 11个品牌的鲨鱼软骨制品 ,每组单一性别 13～ 15只 ,连续给予 18d后 ,每只小鼠左腋下接种 0 2mL浓度为 5× 10 6～ 1× 10 7/mL的S 180细胞 ,继续给予受试物至接种12d后剖杀小鼠 ,取出瘤块 ,称体重、瘤重。同时分别经口灌胃给予Balb C小鼠 0 0 2～ 4 5g kgBW的 11个品牌的鲨鱼软骨制品 ,每组单一性别 13～ 15只 ,连续 30d后 ,测定小鼠免疫指标 (腹腔巨噬细胞吞噬鸡红细胞试验与NK细胞活性测定 )。结果显示 :(1)本实验中对照组小鼠的瘤重为 2 6 0± 0 17g (n =2 4 7只 ) ,受试物组的瘤重为 2 39± 0 15g (n =875只 ) ,抑瘤率仅为 8% ,经成组t检验统计 ,P =0 382 >0 0 5。即所使用的 11个品牌的鲨鱼软骨制品均没有抑制小鼠移植性肉瘤 -180的作用 ;(2 )NK活性实验结果表明 :鲨鱼软骨粉的剂量组与对照组相比 ,差异具有显著性 (P≤0 0 5 )并具有增强作用的占 36 4 % (4 11) ;(3)巨噬细胞吞噬实验结果表明 ,鲨鱼软骨粉剂量组与对照组吞噬率相比差异具有显著性 (P≤ 0 0 5 )并具有增强作用的占 18 2 % (2 11) ;(4 ) 2项免疫实验中至少 1项阳性的占 4 5 4 % (5 11)。 (5 )?The inhibition of shark cartilage on the transplanted sarcoma\|180(S\|180) was assessed for 11 brands of shark cartilage products by testing the growth of S\|180,activity of NK cell and the phagocytosis in mice.The products were given orally to Kunming mice at the dosage (0 02～4 5 g/kg BW) recommended by the manufacturers.The result showed that:(1) The average weight of sarcoma in mice was 2 39±0 15( n =875) in testing groups,and that was 2 60±0 17 ( n =247) in the control group ( P =0 382,>0 05),and the inhibition rate of 8% indicaled that the products had no resistant effect on the growth of sarcoma\|180 in mice;(2) The activity of NK cells was enhanced in only 4 products (36 4%);(3) The phagocytosis was enhanced in only 2 products (18 2%);(4) Only 45 4% of products could enhance more than one of these two immunoreactions;(5) The immunoreaction is more sensitive than the tumour inhibitive activity for shark cartilage.

关 键 词：鲨鱼软骨粉 肉瘤-180 抑制作用 免疫系统 动物实验 肿瘤 治疗 

分 类 号：R73-36[医药卫生—肿瘤]