树鼩、熊猴感染人乙型肝炎病毒肝细胞内感染指征的研究  

Serial pathologic changes in livers of tree shrwes and macaca assamensises infected with human hepatitis B virus

作　　者：王树声[1] 苏建家 冯百芳[3] 李媛 张涛[1] 覃柳亮 黄果勇[1] 高建恩[3] 葛宪民[1] 李河民[4] 

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530021 [2]广西肿瘤研究所 [3]北京大学肝病研究所 [4]中国药品生物制品检定所

出　　处：《中华实验和临床病毒学杂志》2003年第3期244-246,T003,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家"九五"科技攻关课题 (96 90 6 3 0 10 )

摘　　要：目的　对树、熊猴感染人乙型肝炎病毒 (HHBV)后对肝细胞病变进行动态观察。方法　 10只成年树 ,2 8只熊猴接种含人乙型肝炎病毒 (HBV)血清后 ,定期肝活检 ,采用HE染色、免疫组化、原位杂交对实验动物肝组织进行研究分析。结果　 80 %树感染HHBV后 ,通过免疫组化在肝组织内可找到乙型肝炎病毒表面抗原 (HBsAg) ,5 0 %通过原位杂交可检测到HBVDNA。有 2 5 %熊猴的肝组织内可检测到HBsAg ,但信号较弱 ,而肝组织内未发现HBVDNA。结论　树感染HHBV后 ,肝细胞内出现病理改变 。Objective To serially observe the pathologic changes in livers of tree shrews and macaca assamensises infected with HHBV Methods 10 adult tree shrews and 28 macaca assamensises were inoculated with HBV rich human sera The liver of the animals were regularly biopsied The liver samples were examined histopathologically by HE staining Some samples were stained for HBsAg by immunohistochemistry (IH), and HBV DNA by in situ hybridization (ISH) Results HBsAg in 80% of tree shrews infected with HHBV can be detected by IH, HBV DNA in 50% of those can be found by ISH. The positive rates of HBsAg in macaca assamensises ' livers were 25% by IH, none HBV DNA was detected Conclusion The tree shrew model seems to be applicable for the research of human hepatitis B

关 键 词：乙型肝炎病毒 肝细胞 感染指征 树鼩 熊猴 动物模型 免疫组织化学 

分 类 号：R-332[医药卫生]