尿转化生长因子β_1在原发性肝癌中的检测及临床意义  

Clinical significance of transforming growth factor-β_1 in urine of patients with primary hepatic carcinoma.

作　　者：甘雪婷[1] 白宪光[2] 黄团新[1] 吴淑坤[1] 

机构地区：[1]武警湖北总队医院传染科,武汉430061 [2]第四军医大学唐都医院全军感染病诊疗中心,西安710038

出　　处：《武警医学》2003年第9期535-538,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的　探讨尿转化生长因子 β1(TGF - β1)在原发性肝癌患者中的表达及临床意义。方法　采用酶联免疫吸附试验法 (ELISA)测定了 88例原发性肝癌、4 0例肝硬化、4 0例慢性活动性肝炎及 2 0例正常对照者尿中TGF - β1含量。结果　原发性肝癌组患者尿TGF - β1水平明显高于肝硬化组、慢性肝炎组和正常对照组 ,组间的差异具有显著性意义 (P <0 0 1)。以正常对照组患者尿TGF - β1含量 x± 2s为阈值 ,尿TGF - β1诊断原发性肝癌的敏感性为 6 5 9% ,特异性为 74 8%。肿块数目≥3个、直径≥ 3cm、AFP≤ 4 0 0 μg/L的原发性肝癌患者尿TGF - β1水平明显增高。原发性肝癌患者尿TGF - β1水平与血清AFP相关性分析发现 ,r值为 - 0 2 2 ,P <0 0 5 ,提示尿TGF - β1水平与血清AFP存在一定的负相关。 10例原发性肝癌患者介入或手术治疗后尿TGF - β1水平明显下降 (P <0 0 1)。结论　尿TGF -Objective To study the clinical significance of TGF-β 1 in the urine of patients with primary hepatic carcinoma (PHC).Methods TGF-β 1 was determined by ELISA in 88 patients with PHC,40 patients with liver cirrhosis (LC),40 patients with chronic active hepatitis (CAH) and 20 healthy adult controls.Results Urine TGF-β 1 levels in PHC patients were significantly higher than those in healthy controls,LC patients and CAH patients (P<0.01).By using the mean ±2 s of urine TGF-β1 level in healthy controls as the upper limit of normal value (35.6 μg/L),the sensitivity and specificity of urine TGF-β 1 in diagnosis of PHC were 65.9% and 74.8%,respectively.The urine TGF-β 1 levels in 10 cases of PHC significantly decreased after successful anticancer therapy (P<0.01).There was negative correlation between TGF-β 1 and serum α-fetoprotein (AFP)(r=-0.22,P<0.05).Conclusions Urine TGF-β 1 might serve as a novel tumor marker for differential diagnosis and appraisal of therapeutic effect in PHC.

关 键 词：转化生长因子Β1 原发性肝癌 尿液检测 临床意义 酶联免疫吸附试验法 鉴别诊断 

分 类 号：R734.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]