益肺抗瘤饮对实验性肺癌细胞周期及核酸和蛋白质合成的影响  

Effects of Yifei Kangliu Qral Liquid on cell cycle and protein-nucleic acid synthesis of experimental lung cancer

作　　者：韩明权[1] 刘嘉湘[2] 高虹[2] 陈善香[2] 朱晏伟[2] 许玲[3] 

机构地区：[1]上海市肺科医院中西医结合科,上海200433 [2]上海中医药大学龙华医院肿瘤科,上海200032 [3]上海长征医院中医科,上海200003

出　　处：《中西医结合学报》2003年第3期205-208,共4页Journal of Chinese Integrative Medicine

基　　金：上海市青年科技启明星项目 (QB0 31 0 9)

摘　　要：目的　探讨临床验方益肺抗瘤饮对小鼠Lewis肺癌和人肺腺癌SPC A 1细胞增殖和细胞周期及核酸、蛋白质合成的影响。方法　采用体内接种瘤体称重和体外培养细胞计数相结合计算肿瘤抑制率 ,并以流式细胞仪测定肿瘤细胞周期的比例和DNA、RNA及蛋白质指数。结果　实验组肿瘤生长抑制率在 3 0 .3 8% (P <0 .0 5) ,益肺抗瘤饮各组S期细胞比例明显小于对照组 ,最显著的一组有 72 .6%的细胞滞留于G0 /G1期。其中益肺抗瘤饮 3组Lewis肺癌的DNA、RNA和蛋白质合成的抑制率分别为 7.4%、2 3 .73 %和 2 3 .3 1% ,益肺抗瘤饮Ⅱ组SPC A 1细胞DNA、RNA和蛋白质合成抑制率分别为 9.3 %、10 .1%和 14 .7% ,呈逐级放大现象。结论　益肺抗瘤饮以抑制DNA为原始作用 ,抑制Lewis肺癌细胞和SPC A 1细胞进入增殖期 。ObjectiveTo explore the effect of the traditional Ch inese medicine Yifei Kangliu (YFKL) Oral Liquid on the proliferation, cell cycle and protein-nucleic acid synthesis of murine Lewis lung cancer cell and human lung adenocarcinoma cell line SPC-A-1. MethodsThe inhibiting rat es of tumor growth were calculated by weighing the weight of tumor inoculated in vivo, combined by counting cancer cells in vitro. The ratio of the cell cycle and exponents of DNA, RNA, and protein were measured by flow cytometry (F CM). ResultsThe inhibiting rate of tumor growth in the treated gr oup with YFKL Oral Liquid was 30.38% (P <0.05). The proportion of cells in S phase of the treated groups with YFKL Oral Liquid was lower than that of th e control group. In the group with most significant result, 72% of the cells wer e stagnated in G 0/G 1 phase. The inhibiting rates of DNA, RNA and protein in murine Lewis lung cancer were 7.4%, 23.73% and 23.31% respectively. In SPC-A-1 cell line, the inhibiting rates were 9.3%, 10.1% and 14.7% respectively, demons trating amplified effects on lower levels. ConclusionYFKL Oral Liq uid significantly inhibited the proliferation of murine Lewis lung cancer cell a nd human lung adenocarcinoma cell line SPC-A-1 by blocking the cancer cells en tering the proliferative phase resulted from its inhibition of DNA.

关 键 词：益肺抗瘤饮 实验性 肺癌 细胞周期 核酸 蛋白质 

分 类 号：R734.2[医药卫生—肿瘤]