LASIK手术时眼压波动对视神经轴突影响的实验研究  

The effect of intraocular pressure fluctuation during LASIK on rabbit optic nerve axons

作　　者：周浩[1] 褚仁远[1] 周行涛[1] 戴锦晖[1] 孔祥梅[1] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院,上海200031

出　　处：《眼视光学杂志》2003年第3期136-138,共3页Chinese Journal of Optometry & Ophthalmology

基　　金：国家教育部博士点重点基金(20020246081)

摘　　要：目的:定量观察LASIK手术时不同大小的负压吸引对兔视神经轴突的影响,以评估LASIK手术的安全性。方法:33只新西兰兔随机分为常规负压组、高负压组和超高负压组,分别采用不同大小的负压吸引,吸引阶段眼压分别升高至60mmHg、90mmHg和120mmHg,6w后取视神经标本,应用计算机图像分析进行视神经轴突计数。结果:超高负压组手术眼和对照眼视神经轴突数差异有统计学意义,P<0.01;另外两组手术眼与对照眼视神经轴突数差异无统计学意义,P>0.05。结论:在LASIK手术时,不宜采用过高的负压吸引,过高的负压吸引存在着潜在的危险性,瞬间的大幅度非生理性眼压波动可导致视神经轴突的不可逆损害。Objective:LASIK has been widely performed in treating myopia and most operators are unaware of its potential risk to optic nerve fiber. The purpose of the study was to determine quantitatively the effect of intraocular pressure fluctuation during LASEK on rabbit optic nerve axons and evaluate the safety of LASK. Methods: 33 New Zealand white rabbits were divided into regular vacuum group, high vacuum group and ultra-high vacuum group randomly . Different value of vacuum pressure was used during the operation and IOP of three groups were elevated to about 60 mmHg, 90 mmHg and 120 mmHg respectively. After 6 weeks the eyes were enucleated and 1μm cross-sections were made staining with toluidine blue. Photography was taken under light microscope and a computer image analysis system was used to count the optic nerve axons.Results:The optic nerve axon number of control eyes and surgical eyes was 98194±10282, 96151± 11500(regular vacuum group); 92434±10795,89069±8916(high vacuum group), 94071±9348,80953±11548 ( ultra-high vacuum group) respectively. The optic nerve axons of surgical eyes in ultra-high vacuum group are less than the controls( P < 0.01). In the other two groups, there are no significant difference between surgical eyes and controls(P > 0.05). Conclusion: During the LASK, high vacuum suction could be dangerous for eyes. Unphysiologic high IOP flunctuation in a short moment can cause damage to the optic nerve axons.

关 键 词：LASIK手术 眼压波动 视神经轴突 影响 实验研究 

分 类 号：R779.63[医药卫生—眼科]