中脑祖细胞移植治疗大鼠帕金森病  

Transplantation of mesencephalon progenitor cells in treatment of Parkinsonian rats

作　　者：田增民[1] 尹丰[1] 王亚奇[1] 刘爽[1] 周英[1] 

机构地区：[1]海军总医院全军神经外科中心,北京100037

出　　处：《第二军医大学学报》2003年第9期954-956,共3页Academic Journal of Second Military Medical University

基　　金：国家高新技术发展规划 ( 863 )课题( 2 0 0 1AA42 2 110 )

摘　　要：目的 :研究中脑祖细胞作为移植供体治疗帕金森病 (Parkinsondisease,PD)的可行性。 方法 :将SD大鼠制成 6 羟基多巴 (6 OH DA)毁损的PD动物模型 ;从孕龄 1 3d胎鼠的脑中分离出中脑祖细胞 ,在体外进行扩增和诱导分化 ,并移植入PD大鼠纹状体内。 结果 :在碱性成纤维细胞生长因子 (bFGF)和表皮生长因子 (EGF)存在的条件下 ,中脑祖细胞可以在体外稳定扩增 ;白细胞介素 1 (IL 1 )和纹状体上清可以促进中脑祖细胞向多巴胺神经元转化 ;将分化的中脑祖细胞移植入PD大鼠的纹状体内 ,可以明显减轻动物的旋转症状。 结论 :中脑祖细胞移植治疗PD大鼠有一定疗效。Objective:To explore the feasibility of mesencephalon progenitor cells as dopamine neuron's graft in treatment of Parkinson disease(PD) in rats. Methods: Animal model of PD were established in SD rats by 6 hydroxydopamine. Mesencephalon progenitor cells were obtained from 13 day old fetal rats. After in vitro proliferation and differentiation, the cells were planted into PD rat striatum. Results: Progenitor cells were expanded in an undifferentiated state with endothelia growth factor and basic fibroblast growth factor as mitogens. IL 1 and striatal culture media further increased the number of TH immunoreactive cells. Transplantation of these neurons relieved the rotation symptom of PD rats. Conclusion: Transplantation of mesencephalon progenitor cells has certain effects on PD rats.

关 键 词：帕金森病 中脑祖细胞移植 治疗 可行性 6-羟基多巴 

分 类 号：R742.5[医药卫生—神经病学与精神病学]