X刀治疗大鼠C6脑胶质瘤诱发凋亡及抑癌基因p16蛋白的表达  

The induced apoptosis and the p16 Gene's expression in treating rat C6 glioma with X-knife

作　　者：何跃[1] 袁先厚[2] 王伦长[3] 涂汉军[3] 黄宽明[3] 李新建[3] 秦军[3] 王辉[3] 

机构地区：[1]武汉大学医学院 [2]武汉大学中南医院神经外科 [3]十堰市太和医院神经外科

出　　处：《肿瘤防治研究》2003年第5期357-358,412,F002,共4页Cancer Research on Prevention and Treatment

基　　金：湖北省科技厅资助项目 (2 0 0 2A0 30 0 5 )

摘　　要：目的　为了探讨经X刀照射诱导C6脑胶质瘤凋亡及其抑癌基因p16蛋白的表达。方法　建立大鼠C6脑胶质瘤模型 ,经X刀照射后 ,采用TUNEL法检测细胞凋亡 ,免疫组化SABC方法检测抑癌基因p16蛋白表达的变化。结果　治疗组凋亡阳性细胞数明显高于对照组 (P <0 .0 5 ) ;p16基因在蛋白水平表达率也高于对照组 (P <0 .0 5 )。结论　X线可诱导C6脑胶质瘤细胞发生凋亡 ,抑癌基因 p16在辐射诱导凋亡中起重要的作用。Objective To investigate the apoptosis of C6 glioma cell and the p16 gene's protein expression after X-knife radiation. Methods By establishing a rat C6 glioma model,after X-knife, TUNEL and immunohistochemistry technique (SABC methods) was employed to inspect the apoptotic cells and the p16 gene's protein expression respectively. Results X-radiation can induce apoptosis,the apoptosis and the p16 gene's protein expression in the treatment group is higher than that in control group ( P < 0.05 ). Conclusion X-radiation can induce apoptosis in the rat C6 glioma cells, the tumor suppressor gene p16 play an important role in radiation-induced apoptosis.

关 键 词：脑胶质瘤 细胞凋亡 p16基因 立体定向放射治疗 X刀 

分 类 号：R739.41[医药卫生—肿瘤] R730.55[医药卫生—临床医学]