碱性成纤维细胞生长因子和表皮生长因子对成年牛晶状体上皮细胞增殖的影响  

Effects of Basic Fibroblast Growth Factor and Epidermal Growth Factor on Proliferation of Adult Bovine Lens Epithelial Cells

作　　者：胡建石[1] 林玲[1] 郑志竑[1] 朱进伟[1] 林建银[1] 

机构地区：[1]福建医科大学分子医学研究中心,福州350004

出　　处：《福建医科大学学报》2003年第3期289-291,共3页Journal of Fujian Medical University

摘　　要：目的　观察碱性成纤维细胞生长因子 (b FGF)和表皮生长因子 (EGF)对成年牛晶状体上皮细胞增殖的影响。　方法　按正交表 L16 (4 5)进行正交设计 ,将不同浓度 b FGF和 EGF共同作用于体外培养的成年牛晶状体上皮细胞 ,采用 MTT法测定细胞的增殖能力。　结果　单用 b FGF、EGF均可促进成年牛晶状体上皮细胞增殖。0 .75 ng/ m L 的 b FGF开始对晶状体上皮细胞有促增殖作用 ,1～ 10 0 ng/ m L 的 EGF对成年牛晶状体上皮细胞均有明显的促增殖作用 (P<0 .0 1) ,但 b FGF和 EGF无交互作用。　结论　 b FGF和 EGF是诱导成年牛晶状体上皮细胞增殖的重要因素 。Objective\ To explore the effect of basic fibroblast growth factor(bFGF) and epidermal growth factor(EGF) on the proliferation of adult bovine lens epithelial cells.\ Methods\ Different concentration of bFGF or EGF combination according orthogonal design L16(4\+5) were added to adult bovine lens epithelial cells cultured in vitro.\ The proliferative characteristics of the cells were measured with methyl thiazolyl tetrazolium(MTT) assay.\ Results\ 1～100 ng/mL of EGF and above 0 75 ng/mL of bFGF can significantly enhance the proliferation of adult bovine lens epithelial cell(P<0 01).\ But the combination effect of bFGF and EGF was not observed.\ Conclusion\ bFGF and EGF were important factor that can promote proliferation of adult bovine lens epithelial cells.

关 键 词：成纤维细胞生长因子 碱性 表皮生长因子 晶体 上皮细胞 牛 

分 类 号：R776.1[医药卫生—眼科]