高效液相色谱法测定乌梅透骨口服液中阿魏酸的含量  

Quantitative Determination of Ferulic Acid in the Wumeitougu Oral Liquid with HPLC

作　　者：刘高峰[1] 王伟[1] 刘艳[1] 顾凤云[1] 

机构地区：[1]哈尔滨医科大学附属第二医院药学部,哈尔滨市150086

出　　处：《中国药房》2003年第9期554-555,共2页China Pharmacy

基　　金：黑龙江省自然科学基金资助项目(D9806)

摘　　要：目的:研究乌梅透骨口服液中阿魏酸的含量测定方法,比较阿魏酸在不同溶剂中的稳定性。方法:采用反相高效液相色谱法测定。色谱柱为Kromasil C_(18),流动相为甲醇-水(30:70),流速为1ml/min,检测波长为324nm。结果:阿魏酸的线性范围为0.05μg～1.0μg(r=0.9 999),平均回收率为97.01％～101.53％,RSD<1.74％(n=3)。阿魏酸在不含酸混合溶剂(甲醇-水之比为1:4)中更稳定。结论:本方法简便、快速、重现性好,可为该产品的质量控制提供依据。OBJECTIVE: To study the quantitative determination method of ferulic acid in the Wumeitougu oral liquid, compare the stability of ferulic acid in different solvents. METHODS:The detecting conditions of RP -- HPLC were as follow Kromasil C_(18) column(4.6mm×200mm, 5μm); mobile phase: methanol-- water(30:70); flow rate: 1ml/ min: detecting was length: 324nm. RESULTS: The calibration curve revealed linearity in the range of 0.05～1.0μg(r=0.9 999). The mean re? ery was 97.01%～101.53%. RSD<1.74% (n=3). Ferulic acid was more stable in the mixed solvent without acetic acid(m? anol:water was 1:4) .CONCLUSION:The detecting method is simple, rapid and satisfactory in Tepeatability, and can be ? for quality control of Wumeitougu oral liquid.

关 键 词：反相高效液相色谱法 阿魏酸 含量测定 

分 类 号：R927.2[医药卫生—药学]