Smad7基因在细胞恶性转化过程中的促增殖作用  

The Effect of Smad7 Gene on Cell's Proliferation in the Process of Malignant Transformation

作　　者：霍艳英[1] 张开泰[1] 项晓琼[1] 李邦印[1] 徐勤枝[1] 段瑞峰[1] 胡迎春[1] 李刚[1] 吴德昌[1] 

机构地区：[1]军事医学科学院放射医学研究所,中国北京100850

出　　处：《生命科学研究》2003年第3期224-227,共4页Life Science Research

基　　金：国家重点基础研究发展规划973项目(G1998051207)

摘　　要：用基因转染的方法,建立稳定表达Smad7基因的永生化及恶性化人支气管上皮细胞系,用MTT法检测Smad7基因过表达对细胞生长、增殖的影响及对TGF β1介导的生长抑制效应的影响.结果表明在永生化和恶性化人支气管上皮细胞系BEP2D和BERP35T2中各筛选到2个稳定表达SMAD7蛋白的克隆,命名为BS7 1、BS7 2(来源于BEP2D),RS7 1、RS7 2(来源于BERP35T2).这些细胞系细胞的生长、增殖能力均强于转染空载体的对照细胞系,同时TGF β1对这些细胞系的生长抑制能力明显减弱.提示Smad7基因通过降低细胞对TGF β的应答来促进细胞的生长、增殖能力.Gene transfection and MTT were performed to determine the effect of Smad7 on cells growth and proliferation. The results showed that subclonal BEP2D and BERP35T2 cell lines BS71, BS72, RS71 and RS72 which constitutively express SMAD7 were established. Overexpression of SMAD7 resulted in marked increase in the proliferation of these cell lines and loss of the growth inhibitory response to TGFβ1. It could be concluded that the overexpression of Smad7 may facilitate cells proliferation and contribute to carcinogenesis by antagonizing TGFβmediated growth inhibition.

关 键 词：SMAD7基因 细胞恶性转化 促增殖作用 真核表达 

分 类 号：Q253[生物学—细胞生物学] R730[医药卫生—肿瘤]