^(99m)TcN-MIBI在体外肿瘤细胞内的摄取及其与^(99m)Tc-MIBI的比较研究  

Uptake of ^(99m)TcN-MIBI in Tumor Cells in vitro and the Comparison of it with ^(99m)Tc-MIBI

作　　者：张现忠[1] 田彦聪[1] 王学斌[1] 罗茜[1] 

机构地区：[1]北京师范大学化学系,北京100875

出　　处：《高等学校化学学报》2003年第10期1742-1746,共5页Chemical Journal of Chinese Universities

基　　金：北京师范大学青年科学基金 (理科 ) (批准号 :10 7195 )资助

摘　　要：研究了结肠癌细胞 CL-1 87、肺腺癌细胞 L-973以及乳腺癌细胞 MCF-7分别对 99m Tc N-MIBI和99m Tc-MIBI配合物的摄取情况 .结果表明 ,99m Tc N-MIBI在 3种肿瘤细胞内培养 2 0～ 40 min时的摄取达到极大值 ,并且摄取量明显高于 99m Tc-MIBI,特别是在结肠癌细胞 CL-1 87中 .99m Tc N-MIBI在乳腺癌细胞 MCF-7内的摄取量最高 ,培养 40 min时的单位细胞摄取百分率约为 3 0 % /1 0 5个细胞 ;在结肠癌细胞 CL-1 87内的摄取量次之 ;在肺腺癌细胞 L-973内的摄取量最低 .细胞摄取条件实验结果表明 :细胞浓度和培养温度的变化均会对细胞摄取产生影响 .In this study, the uptake of 99mTcN-MIBI and 99mTc-MIBI were investigated by colorectal cancer cell line CL-187, lung cancer cell line L-973 and breast cancer cell line MCF-7 in vitro, respectively. The results of uptake kinetics shows that 99mTcN-MIBI has maximal accumulations between 20 and 40 min after incubation in all of these tumor cells, and the accumulation(D%) of 99mTcN-MIBI was found to be significantly higher than that of 99mTc-MIBI, especially in colorectal cancer cell line CL-187. Uptake of { 99mTcN-}MIBI decreased in the order MCF-7→CL-187→L-973. The maximal accumulation occurring at 40 min after incubation of 99mTcN-MIBI in MCF-7 with the uptake rate about 30% per 10 5 cells. The influences of the culture conditions, such as cell concentration and incubation temperature, on the accumulation were also investigated, and the uptake of 99mTc-complexes by cells were affected significantly by the culture conditions.

关 键 词：^99mTcN—MIBI 体外肿瘤细胞 ^99MTC-MIBI 比较研究 放射性药物 中性脂溶性肿瘤显像剂 

分 类 号：R730.44[医药卫生—肿瘤] R981[医药卫生—临床医学]