盐胁迫下小麦耐盐突变体后代差异cDNA的分离和鉴定  

Isolation and Characterization of the cDNA Fragments of Wheat Involved in Salt Stress

作　　者：陈桂平[1] 黄占景[1] 马闻师[1] 沈银柱[1] 

机构地区：[1]河北师范大学生命科学学院

出　　处：《中国农业科学》2003年第9期996-999,共4页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 471);河北省自然科学基金资助项目 ( 3 0 110 3 )

摘　　要：采用cDNA AFLP(cDNA amplifiedfragmentlengthpolymorphism)技术分析了遗传背景相近的小麦后代突变体中 ,耐盐性较强的RH870 6 4 9(saltresistant,SR)和盐敏感的H870 6 34(saltsensitive ,SS)在盐胁迫和非胁迫情况下基因表达的差异。结果表明 ,其中 88.1%的条带在 4个样品中是一致的 ,只有 11.9%的条带在 4个样品中表现出差异。选取其中的 6 8个差异条带进行克隆 ,测序 35个 ,经Internet进行Blast比较 ,发现 11个片段 (31.4 % )与已知基因具有较高的同源性 ,主要涉及与离子转运有关的蛋白、与信号转导有关的蛋白以及与氧化胁迫有关的蛋白 ;另外2 4个片段 (6 8.6 % )与已知基因同源性较低或未发现同源性 ,可能是一些未知基因。cDNA-AFLP(amplified fragment length polymorphism) was used to isolate genes differentially expressed in salt-stressed and unstressed wheat lines RH8706-49 and H8706-34 derived from a single seed. RH 8706-49 is a salt-stress resistant (SR) line and H8706-34 is a salt-stress sensitive (SS) line. About 88.1% of the cDNA fragments were expressed in all the four samples, 11.9% were different among the samples. Sixty-eight cDNA fragments were cloned, 35 of them were subject to sequence analysis. Database searches indicate that 11 cDNA fragments show high homology to known genes, which mainly include proteins related to ion transport, signal transduction and oxidative stress. The remaining 24 cDNA show no detectable homology to known genes, suggesting that they probably represent novel genes.

关 键 词：盐胁迫 小麦 耐盐突变体 后代差异 CDNA 分离鉴定 克隆 基因测序 

分 类 号：S512.1[农业科学—作物学]