大豆胞囊线虫生理小种的鉴定及其比较电泳研究  

Races identification and its SDS--PAGE of Heterodera glycines in China

作　　者：齐军山[1] 陈品三[2] 胡起宇[3] 王寿华[4] 

机构地区：[1]山东省农科院植保所,山东济南250100 [2]中国农科院植保所,北京100094 [3]南昌大学生物系,江西南昌330047 [4]中国农业大学植病系,北京100094

出　　处：《中国油料作物学报》2003年第3期71-75,共5页Chinese Journal of Oil Crop Sciences

基　　金：山东省自然科学基金资助项目(Z99D01)

摘　　要：对采自内蒙古、山东、黑龙江、江苏、北京五省市(区)大豆胞囊线虫种群在国际统一的鉴别寄主Pickett、Peking、PI88788、PI90763和Lee上进行了生理小种鉴定。鉴定出1号、2号、3号、4号、5号和6号生理小种。其中1号、3号和4号生理小种分布较广,为优势生理小种。应用改良的SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)将大豆胞囊线虫1～4号生理小种中国种群的可溶性蛋白分为30～31条蛋白带。其中3号和4号生理小种有31条蛋白带,而1号和2号生理小种只有30条蛋白带,前者比后者多一条蛋白带,其分子量为91201。Twenty six isolates of Heterodera glycines from five provinces in China were tested on standard soy- bean race differentials. A total of 6 variants classified as races 1 -- 6 were found. However, race 1,3 and 4 were still principal races in China. Modified SDS -- polyacrylamide gel electrophoresis (SDS -- PAGE) was used to sepa- rate Heterodera glycines races. The banding patterns of soluble proteins from H. glycines races 1～4 showed that the SDS -- PAGE system resolved 30～31 bands for races 1～4. A protein band unique to H. glycines races 3 and 4 was not detected in SDS -- polyacrylamide gel profiles from races 1 and 2. Molecular weight determination was 91201 for distinctive proteins in profiles of H. glycines races 3 and 4.

关 键 词：大豆 胞囊线虫 生理小种 鉴定 SDS-PAGE 鉴别寄主反应 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]