怀槐培养细胞中异黄酮分析及其保肝作用  

Study on Extraction and Hepatoprotective Function of Isoflavones from Callus Cultures of Maackia amurensis

作　　者：罗建平[1] 沈国栋[1] 姜绍通[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,合肥230009

出　　处：《食品科学》2003年第10期139-142,共4页Food Science

基　　金：国家自然科学基金资助项目(30170059)

摘　　要：怀槐愈伤组织具有异黄酮合成能力,HPLC分析表明培养3周的愈伤组织中积累芒柄花黄素、染料木素、大豆甙元等三种主要异黄酮含量分别平均为53.4、12.3、17.4μg/g FW。愈伤组织经乙醇提取和乙酸乙酯萃取可有效去除杂质,萃取出的总异黄酮对小鼠四氯化碳急性肝损伤有显著的保护作用,可以有效降低肝损伤引起的血清GOT、GPT升高,增加血清TP、Alb的含量,效果均好于甘草甜素片。Callus cultures induced from hypocotyl and cotyledon segments of Maackia amurensis have had the capacity forbiosynthesis of isoflavones. HPLC analysis showed that the callus cultures have accumulated formononetin, genistein and daidzein,the three representative isoflavones found in the heartwood of M.amurensis. The average contents of formononetin, genisteinand daidzein in callus cultured for 3 weeks were 53.4, 12.3 and 17.4μg/g cell fresh weight, respectively. Extraction of isoflavonesby ethyl alcohol and ethyl acetate from callus cultures was an effectively impurity-reducing method. Total isoflavones fromcallus cultures have had a remarkable hepatoprotective effect against acute CCl4-hepatitis in mice. Its hepatoprotective effectsof attenuating the activities of serum aminotransferases (GOT and GPT) and elevating the content of serum albumin (Alb) werebetter than those of the glycyrrhizin tablets.

关 键 词：怀槐 愈伤组织 异黄酮 保肝作用 药用 

分 类 号：R285[医药卫生—中药学] R282.71[医药卫生—中医学]