视网膜新生血管动物模型的制备  

Preparation of animal model of high oxygen inducing retinal neovascularizaton

作　　者：曹晖[1] 胡宏慧[1] 许迅[1] 樊莹[1] 王方[1] 张皙[1] 

机构地区：[1]上海交通大学附属第一人民医院眼科,200080

出　　处：《眼科新进展》2003年第5期335-337,共3页Recent Advances in Ophthalmology

摘　　要：目的　制备视网膜新生血管动物模型 ,为今后的视网膜新生血管相关疾病的研究提供稳定的模型。方法　以出生 7d的C5 7BL/ 6J小鼠 5 6只 ,雌雄兼有 ,随机将 2 8只放入75 %± 2 %高氧环境 ,控制室温 2 3℃± 2℃ ,日光照明 ,5d后返回空气环境 ;另一组 2 8只置于 2 3℃± 2℃空气环境中饲养作为对照。随机于出生后 12、14、17、2 1、2 2、2 5d取高氧组和空气组小鼠行视网膜铺片、血管内皮生长因子 (vascularendothe lialgrowthfactor ,VEGF)免疫组化染色 ,观察VEGF的表达情况 ,并对出生后 17d小鼠的视网膜铺片、石蜡切片HE染色、VEGF免疫组化染色。结果　高氧诱导组出生后 17d视网膜无血管区面积 ,穿过视网膜内界膜细胞核计数明显高于空气组。血管内皮细胞VEGF的表达从出生后 14d开始有阳性表达 ,阳性表达逐渐增强 ,出生后 17d达到高峰 ,之后逐渐下降 ,持续至出生后 2 1d。结论　该模型为一种合适的视网膜新生血管动物模型。Objective To develop proliferative neovascularizat ion in the mouse for researching therapeutic intervention of retinal neovascularization in the retinopathy. Methods A total of 56 one week old neonatal C57BL/6J mice were included in this study.Twenty eight ofthem were randomly selected to be exposed to 75%±2% oxygen environment and the temperature was controlled at 23℃±2℃. These mice were back to natural air environment after 5 days. The other 28 mice, designed as control group, were fed only in natural air environment. Vascular endothelial growth factor(VEGF)expression was observed at 12, 14, 17, 21, 22 and 25 days after birth in both exposed group and control group by means of retina flat mounting and VEGF immunohistochemical staining. The retina of each 17 day old mouse was dissected, HE stained and immunohistochemically stained.Results The avascular area of retinal and the number of nuclei extending from retinal area into vitreous of 17 day old mice in exposed group were significantly higher than those of the control group. VEGF expression of endothelium continuously increased since the 14th day after birth and showed a peak at the 17th day, then gradually decreased until the end of observation.Conclusion This mouse model of oxygen induced retinal neovascularization should be useful for the study of retinal neovascularization.

关 键 词：视网膜 新生血管 血管内皮生长因子 

分 类 号：R364.3[医药卫生—病理学] R774.1[医药卫生—基础医学]